摘要
目的本文旨在鉴定福建省2016年冬季导致病毒性胃肠炎暴发的病原体,并对病原体进行分子特征研究。方法对疫情暴发地上送的福建省2016年胃肠炎暴发急性病例标本,采用荧光PCR初步判定病原体,常规RT-PCR检测诺如病毒RNA聚合酶和衣壳蛋白基因片段,并进行序列测定和分子特征分析。结果在3起暴发疫情中18份标本经荧光PCR检测均为诺如病毒核酸阳性,其中15份标本RT-PCR检测判为GII型诺如病毒,9份标本成功测序。分析测序结果,证实引起本轮疫情为诺如病毒新重组株,该重组株有别于本地散发流行及全球暴发流行的优势基因型GII.4。毒株RNA聚合酶核苷酸序列与2016年日本的GII.4悉尼变异株Kawasaki194毒株的同源性最高,达98%,为GII.P16亚型;衣壳蛋白核苷酸序列与2008年比利时的IPH2161-08VG06毒株同源性最高(97.7%~98.8%),为GII.2亚型。结论这是福建省首次报道诺如病毒重组株GII.P16/GII.2的检出,并引起病毒性胃肠炎暴发。
We delineated the molecular characteristic of recombinant strain GII. P16/GII. 2 of norovirus associated with acute viral gastroenteritis outbreaks in Fujian Province in winter of 2016. Norovirus were detected in specimens of patients col- lected from the gastroenteritis outbreaks by real-time reverse transcription-PCR and reverse transcription-PCR （RT-PCR）. The PCR products of the positive samples were purified, and partial RNA dependent RNA polymerase （RdRp） gene and partial cap- sid gene were sequenced. The sequences were analyzed using bioinformatics software and online database,and phylogenetic tree were also constructed. Norovirus were detected in all 18 stools. Analysis of 9 positive sequences indicated an emergence of norovirus GII. P16/ GII. 2 and confirmed being the cause of gastroenteritis outbreaks. All the strains shared homology of 98% with strains of Kawasaki 194 of Japan detected in 2016 and 97.7%- 98. 8% with IPH2161-08VG06 of Belgium detected in 2008, RdRp and capsid separately. These outbreak strains showed some degree of differences from the predominant strain, 2012 Sydney GII. 4 variant. This is the first time to have found norovirus GII. P16/ GII. 2 causing viral gastroenteritis out- breaks in Fujian. More in-depth analysis of the Norovirus GII. P16/ GII. 2 could be useful to optimize preventative strategies and develop new and more effective therapeutic measure.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2017年第9期805-808,813,共5页
Chinese Journal of Zoonoses
基金
国家高技术研究发展计划(2011AA02A114)~~
