摘要
目的探讨龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞的保护作用。方法将40只SD大鼠随机分为5组,空白对照组(Control组)、假手术组(Sham组)、心肌缺血再灌注损伤组(MIRI组)、假手术+SDF组(SDF组)、MIRI+SDF组(MIRI&SDF组),每组8只。采用结扎左冠状动脉前降支30 min再灌注2 h的方法建立大鼠MIRI模型,于术前1周开始灌药。采用比色法测定血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)水平,采用TTC染色评估心肌坏死面积,用TUNEL法检测心肌细胞凋亡率。结果与Control、Sham和SDF组比较,MIRI组、MIRI&SDF组的CK、LDH、CK-MB的水平均明显升高(P<0.05)。与MIRI组比较,MIRI&SDF组的CK、LDH、CK-MB的水平明显下降(P<0.05)。MIRI&SDF组心肌梗死面积和心肌细胞的凋亡率明显小于MIRI组(P<0.05)。结论龙血竭总黄酮对MIRI大鼠心肌细胞具有较好的保护作用。
Objective To investigate the protective effect of Sanguis Draconis flavones（SDF）on myocardial cells in rats with myocardial ischemia-reperfusion injury（MIRI）.Methods 40 SD rats were randomly divided into five groups： control group, sham group, MIRI group, sham＋SDF group and MIRI＋SDF group, with 8 cases in each group. The MIRI model was established by ligating left anterior descending coronary artery for 30 minutes and reperfusing for 2 hours. The drugs were administered one week before operation. The levels of serum creatine kinase（CK）, creatine kinase isoenzyme MB（CK-MB） and lactic dehydrogenase（LDH） were measured by colorimetric assay. The area of myocardial necrosis was assessed by triphenyltetrazolium chloride（TTC） staining, and the apoptosis rate of cardiomyocytes was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling（TUNEL）.Results Compared with those in the control group, sham group and the sham＋SDF group, the levels of CK, LDH and CK-MB in the MIRI group and the MIRI＋SDF group were significantly higher（P〈0.05）, while compared with those in the MIRI group, the levels of CK, LDH and CK-MB in the MIRI＋SDF group were significantly lower（P〈0.05）. The area of myocardial infarction in the MIRI＋SDF group was significantly smaller than that in the MIRI group（P〈0.05）, and the apoptotic rate of myocardial cells in the MIRI＋SDF group was significantly smaller than that in the MIRI group（P〈0.05）.Conclusion Sanguis Draconis flavones can effectively protect the myocardial cells of rats with myocardial ischemia-reperfusion injury.
出处
《中国临床新医学》
2017年第9期857-860,共4页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
广西自然科学基金项目(编号:2010GXNSFA013269)
广西中医药管理局科研项目(编号:GZZC14-63)
关键词
龙血竭总黄酮
心肌缺血再灌注损伤
细胞凋亡
Sanguis Draconis flavones（SDF）
Myocardial ischemia-reperfusion injury（MIRI）
Cell ap-optosis
