摘要
采用液相色谱-串联质谱仪(LC-MS/MS)建立双孢蘑菇(Agaricus bisporus)培养料中四环素、二甲胺四环素、土霉素、甲烯土霉素、脱氧土霉素、金霉素、去甲基金霉素7种四环素类药物残留的定量分析方法。双孢蘑菇培养料以等体积Na2EDTA McIlvaine缓冲溶液(pH 4.0)和甲醇的混合液涡动,超声提取(35KHz,5min)。以HLB固相萃取柱进行净化富集,LC-MS/MS检测,结果表明:在5.0~200.0μg/L的线性范围内,7种四环素类药物的峰面积与其浓度呈良好线性关系,方法最低检出限为5.0μg/kg;在5.0、20.0、100.0μg/kg三个添加水平下,培养料基质中7种四环素类药物的回收率为80.3%~92.5%,相对标准偏差为1.6%~8.0%。该方法可以同时满足双孢蘑菇培养料中7种四环素类药物残留检测的需要。
A quantitative analysis method was established for detecting 7 residual tetracyclines（tetracycline,minocycline,oxytetracycline,metacycline,doxycycline,chlortetracycline,and demeclocycline）in Agaricus bisporus compost by LC-MS/MS.Compost was first extracted twice in a mixture of Na2 EDTA McIlvaine buffer（pH 4.0）and methanol（1∶1,v∶v）by vortex and ultrasonic,and then extracts were concentrated by a HLB solid-phase extraction column before LC-MS/MS analysis.The results turned out that under a concentration range of 5.0~200.0μg/L,the integration areas of 7 tetracycline antibiotics were in good linear correlation with their corresponding concentrations,and the detection limit was 5.0μg/kg.The recovery rates of 7 tetracyclines ranged between 80.3% and 92.5% with relative standard deviations between1.6% and 8.0% under concentrations at 5.0,20.0 and 100.0μg/kg.
出处
《食用菌学报》
CSCD
北大核心
2017年第3期63-67,共5页
Acta Edulis Fungi
基金
上海市市级农口系统青年人才成长计划[沪农青字(2016)第1-25号]
上海市农委科技兴农重点攻关项目[沪农科攻字(2016)第4-5号和沪农科攻字(2016)第4-6号]资助
关键词
双孢蘑菇
残留量
定量分析
Agaricus bisporus
drug residue
quantitative analysis
