摘要
Objective： We aimed to investigate protective effects ofvit E on oxidative stress status and homocysteine （Hey） in cardiac tissue of diabetic rats. Methods： Sixteen Wistar male rats were treated with STZ （streptozotocin） （60 mg/kg） to induce diabetes. Diabetic rats were divided into two groups： NTD （non-treated diabetic） and VETD （vit E-treated diabetic） rats. The VETD group received 300 mg/kg vit E with daily feeding. Eight normal rats of the same age were used as the control group. After 6 weeks, the rats were anesthetized, their cardiac tissue was removed, and homogenated supernatant was separated. Samples were assayed for TAC （total antioxidant capacity）, LPO （lipid peroxidation）, nitrite （NO2）, nitrate （NO3）, and Hcy. Key Findings： The contents of LPO, NO3 and Hcy in NTD compared to control group indicate a significant increase, but the levels of these parameters decreased in VETD （p 〈 0.05）. There was a significant decrease in the amount of TAC in the NTD group but in VETD group, that significantly increased （p 〈 0.05）. The amount of NO2 in NTD and VETD groups, compared to the control group, did not show any significant changes （p 〉 0.05）. Conclusions： Significant decrease of oxidative stress and Hey in the cardiac tissue caused by vit E supplementation strongly indicated that this radical scavenger may promote a protective effect on diabetic cardiomyopathy through the attenuation of oxidative stress and increase antioxidant defense mechanism.
