摘要
芸薹生链格孢侵染导致的黑斑病是十字花科蔬菜的重要病害,常造成严重的经济损失。其分生孢子对于病害的侵染循环起着重要作用,但目前对于芸薹生链格孢的产孢机制及产孢相关基因的研究较少。本研究从构建的芸薹生链格孢转化子库中筛选得到了9株产孢缺陷突变体,发现其在菌丝生长形态和孢子产量等方面明显异于野生菌。利用TAIL-PCR技术确定突变体M37的插入位点侧翼序列,研究表明,插入位点为芸薹生链格孢一个G蛋白α亚基基因(Ab G1)编码区。该研究将有助于芸薹生链格孢产孢基因和产孢机制的相关研究。
Black spot disease,caused by Alternaria brassicicola,is one of the most important diseases of cruciferous vegetables and causes serious economic losses.The conidia play an important role in disease cycle,but the study on conidiation mechanism and related genes in A.brassicicola are seldom.In this research,nine conidiation-deficiency mutants were obtained from the transformants.The growth rate,colony morphology and conidiation were significantly different from the wildtype strain.The insertion site flanking sequence was amplified by TAIL-PCR and encoded a Gα subunit gene of A.brassicicola.These results would be helpful for the research on conidiation mechanism and related genes.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第9期3831-3836,共6页
Genomics and Applied Biology
基金
国家自然科学基金(No.31301858)
山东省自然基金(No.81673542和ZR2015CL006)
山东省现代农业产业技术体系创新团队资金共同资助
