摘要
本试验旨在探究绿茶粉及绿茶多酚对犬的抗氧化作用。选用5月龄杂交幼犬15只,随机分为3个组,每组5只。对照组饲喂基础饲粮,绿茶粉组饲喂基础饲粮+1.0%的绿茶粉,绿茶多酚组饲喂基础饲粮+0.25%的绿茶多酚。试验期为84 d。结果表明:与试验开始时相比,试验结束时对照组犬体重增长了162%,绿茶粉组犬体重增长了101%,绿茶多酚组犬体重增长了132%,且绿茶粉组和绿茶多酚组试验结束时犬体重显著低于对照组(P<0.05),比对照组分别降低了61%和30%。试验第84天,绿茶粉组及绿茶多酚组犬血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性均显著或极显著低于对照组(P<0.05或P<0.01),血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性极显著高于对照组(P<0.01),血清丙二醛(MDA)含量极显著低于对照组(P<0.01),肝脏抗氧化基因[谷氨酸半胱氨酸连接酶催化亚基(GCLC)、谷氨酸半胱氨酸连接酶修饰亚基(GCLM)、血红素氧合酶1(HO-1)、过氧化氢酶(CAT)]和Ⅱ相解毒酶基因[谷胱甘肽硫转移酶M1(GSTM1)和醌氧化还原酶1(NQO1)]的mRNA相对表达量显著高于对照组(P<0.05)。综上所述,在本试验条件下,在犬饲粮中添加1.0%的绿茶粉或0.25%的绿茶多酚均具有保护肝脏、抗氧化、促进Ⅱ相解毒酶基因表达及控制体重的作用。
The present study was conducted to investigate the antioxidant effects of green tea powder and green tea polyphenols （GPTs） on canines. Fifteen 5-month old canines were selected and randomly divided into three groups （5 canines in each group）. The canines in control group were fed a basal d ie t, the canines in green tea power group were fed the basal diet plus 1.00% green tea power, and the canines in GPTs group were fed the basal diet plus 0.25%GPTs. The feeding experiment lasted for 84 days. The results showed as follow s; 1） compared with the initial body weight, the final body weight of control group, green tea power group and GTPs group was increased by 162% , 101% and 132% , respectively. The final body weight of green tea power group and GTPs group was significantly lower than that in control group （P 〈0.05） , which was decreased by 61% and 30% compare with control group, respectively. On the 84th day of experiment, the activities of ser-um alanine transaminase （ ALT ） , aspartate aminotransferase （ A S T ） and lactic dehydrogenase （ L D H ） in green tea power group and GTPs group were significantly or extremely significantly lower than those in control group （P 〈0.05 or P 〈0.01） , the activities of serum glutathione peroxidase （GSH-P x） and superoxide dis- mutase （SOD） were extremely significantly higher than those in control group （P 〈0.01） , the content of ser-um malondialdehyde （MDA ） was extremely significantly lower than that in control group （P 〈0.01） , and the relative mRNA expression levels of antioxidant genes [ glutamate cysteine ligase catalytic subunit （ GCLC） , glutamate cysteine ligase subunit modification （ GCLM） , heme oxygenase 1 （HO-1） and catalase （ CAT） ] and phase II detoxification genes [ quinone oxidoreductase 1 （NQOl） and glutathione S transferase M 1 （GSTM1） ] in liver were significantly higher than those in control group （P 〈0.01）. In summary, adding 1.00% green tea powder or 0.25% GTPs in the diet o f canines have the effects of liver protection, antioxidant, activating phase II detoxification gene expression and controlling body weight under the condition of the pres-ent study.
出处
《动物营养学报》
CAS
CSCD
北大核心
2017年第10期3737-3749,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
2016年度安徽省博士后研究人员科研活动经费资助项目(2016B117)
现代农业产业技术体系资助(CARS-19)
茶叶化学与健康领军人才团队引进重大示范项目
国家重点研发计划(2016YFD0501009)
关键词
犬
绿茶粉
绿茶多酚
肝功能
抗氧化基因
n相解毒酶基因
canines
green tea powder
green tea polyphenols
liver funct ion
antioxidant genes
phase II detoxification genes
