摘要
目的建立同时测定鼻敏感颗粒中盐酸麻黄碱、盐酸伪麻黄碱、甘草苷、肉桂酸、毛蕊异黄酮、甘草酸、6-姜辣素、五味子醇甲、五味子醇乙、五味子甲素、五味子乙素的方法。方法采用Hedera-C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,流速1.0mL·min^(-1),检测波长210 nm,柱温30℃。结果盐酸麻黄碱、盐酸伪麻黄碱、甘草苷、肉桂酸、毛蕊异黄酮、甘草酸、6-姜辣素、五味子醇甲、五味子醇乙、五味子甲素、五味子乙素线性范围分别为0.6175~19.76μg·mL^(-1)(r=0.9999)、0.6494~20.78μg·mL^(-1)(r=0.9999)、0.2142~13.71μg·mL^(-1)(r=0.9999)、0.082 40~5.275μg·mL^(-1)(r=0.9999)、0.1558~9.970μg·mL^(-1)(r=0.9999)、1.888~60.42μg·mL^(-1)(r=0.9999)、0.1581~10.12μg·mL^(-1)(r=0.9999)、0.1616~10.34μg·mL^(-1)(r=0.9999)、0.077 80~4.980μg·mL^(-1)(r=0.9999)、0.080 00~5.120μg·mL^(-1)(r=0.9999)、0.082 90~5.305μg·mL^(-1)(r=0.9999),平均回收率分别为101.7%(RSD=2.3%)、102.2%(RSD=1.7%)、98.9%(RSD=2.7%)、100.0%(RSD=2.4%)、98.9%(RSD=1.6%)、102.0%(RSD=2.7%)、100.0%(RSD=2.4%)、99.4%(RSD=2.6%)、100.3%(RSD=1.9%)、101.5%(RSD=1.3%)、101.3%(RSD=1.2%)。结论本试验建立的鼻敏感颗粒的多成分测定方法可靠,重复性好,可用于鼻敏感颗粒的质量控制。
Objective To determine 11 components（ephedrine hydrochloride, pseudoephedrine hydrochloride, liquiritin, cinnamic acid, calycosin, glycyrrhizic acid, 6-gingerol, schisandrin, schisandrol, schizandrin, and schisandrin B） in Bimingan granules simultaneously. Methods The determination was performed on a Hedera C（18）（4.6 mm×250 mm, 5 μm） chromatographic column, and the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution with gradient elution at a flow rate of 1.0 mL·min^（-1）; the detection wavelength was 210 nm, and the column temperature was 30 ℃. Results The calibration curves of ephedrine hydrochloride, pseudoephedrine hydrochloride, liquiritin, cinnamic acid, calycosin, glycyrrhizic acid, 6-gingerol, schisandrin, schisandrol, schizandrin, and schisandrin B were linear at 0.6175-19.76 μg·mL^（-1）（r = 0.9999）, 0.6494-20.78 μg·mL^（-1）（r = 0.9999）, 0.2142-13.71 μg·mL^（-1）（r = 0.9999）, 0.082 40-5.275 μg·mL^（-1）（r = 0.9999）, 0.1558-9.970 μg·mL^（-1）（r = 0.9999）, 1.888-60.42 μg·mL^（-1）（r = 0.9999）, 0.1581-10.12 μg·mL^（-1）（r= 0.9999）, 0.1616-10.34 μg·mL^（-1）（r = 0.9999）, 0.077 80-4.980 μg·mL^（-1）（r = 0.9999）, 0.080 00-5.120 μg·mL^（-1）（r = 0.9999）, and 0.082 90-5.305 μg·mL^（-1）（r = 0.9999）. The average recoveries were 101.7%（RSD = 2.3%）, 102.2%（RSD = 1.7%）, 98.9%（RSD = 2.7%）, 100.0%（RSD = 2.4%）, 98.9%（RSD = 1.6%）, 102.0%（RSD = 2.7%）, 100.0%（RSD = 2.4%）, 99.4%（RSD = 2.6%）, 100.3%（RSD = 1.9%）, 101.5%（RSD= 1.3%）, and 101.3%（RSD = 1.2%）. Conclusion The established method is reliable and reproducible, which can be used for the quality control of Bimingan granules.
作者
张先林
陈慧
邹静
彭懿
刘志辉
ZHANG Xian-lin CHEN Hui ZOU Jing PENG Yi LIU Zhi-hui(The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210046 Nanjing University of Chinese Medicine, Nanjing 210029)
出处
《中南药学》
CAS
2017年第9期1280-1285,共6页
Central South Pharmacy
基金
2010年度"康缘中医药科技创新与奖励基金"专项课题(No.HZ1001KY)
