摘要
目的:探讨葛根素对成骨细胞增殖及骨形态发生蛋白的影响。方法:采用血清药理学方法制备葛根素含药血清,分为葛根素组、阳性对照组和阴性对照组。从SD大鼠乳鼠颅骨获取成骨细胞,分别用含药血清培养分离得到的成骨细胞。采用CCK-8检测成骨细胞增殖、酶联免疫吸附试验检测骨形态发生蛋白2(BMP-2)的表达;采用微量酶标法检测碱性磷酸酶的活性。结果药物作用24、48和72 h后,葛根素组D值显著高于阴性对照组(P<0.05),且显著低于阳性对照组(P<0.05)。随着时间的增加,葛根素组和阳性对照组D值在药物作用48 h达到最高,至72 h后有下降趋势。药物作用3 d和7 d后,葛根素组BMP-2表达水平均显著高于阴性对照组(P<0.05),且显著低于阳性对照组(P<0.05)。药物作用3d和7 d后,葛根素组BMP-2表达水平均显著高于阴性对照组(P<0.05),且显著低于阳性对照组(P<0.05)。药物作用3 d和7 d后,葛根素组碱性磷酸酶活性均显著高于阴性对照组(P<0.05),且显著低于阳性对照组(P<0.05)。结论葛根素可能通过诱导BMP-2表达促进增殖,活化碱性磷酸酶活性促进生物矿化。
Objective： To investigate the effect of puerarin on osteoblast proliferation and bone morphogenetic protein. Methods：The serum containing puerarin was prepared by serum pharmacological method,and divided into puerarin group,positive control group and negative control group. Osteoblasts were extracted from SD rat skull,and osteoblasts were cultured with corresponding serum. CCK-8 was used to detect osteoblast proliferation. The expression of bone morphogenetic protein 2（ BMP-2） was detected by enzyme-linked immunosorbent assay（ ELISA）. The activity of alkaline phosphatase was detected by micro-enzyme method. Results： The values of D in puerarin group were significantly higher than those of the negative control group at 24,48 and 72 hours（ P〈 0. 05）,and were significantly lower than those in positive control group（ P 〈 0. 05）. With the increase of time,the D value of puerarin group and positive control group reached the highest at 48 h,and decreased after 72 h. The expression of BMP-2 in puerarin group was significantly higher than that of the negative control group（ P 〈 0. 05）,and was significantly lower than that in positive control group（ P 〈 0. 05）. The expression of BMP-2 in puerarin group was significantly higher than that of the negative control group（ P 〈 0. 05）,and was significantly lower than that of the positive control group（ P 〈 0. 05）. The activity of alkaline phosphatase in puerarin group was significantly higher than that of the negative control group（ P 〈 0. 05）,and significantly lower than that of the positive control group（ P 〈 0. 05）. Conclusion： Puerarin may promote the proliferation and activate alkaline phosphatase activity by promoting BMP-2 expression to promote biomineralization.
作者
路立花
杨莉
徐玉荣
Lu Lihua Yang Li Xu Yurong(Department of Somatology of Hebei Eye Hospital, Xingtai 054000, Chin)
出处
《世界中医药》
CAS
2017年第9期2139-2142,共4页
World Chinese Medicine
基金
河北省科技计划项目(14277795D)
