摘要
目的研究紧密连接蛋白在6-羟多巴(6-OHDA)制备的帕金森病(PD)大鼠模型十二指肠黏膜的表达变化。方法用6-OHDA损毁双侧中枢黑质多巴胺能神经元建立大鼠模型。用免疫荧光组织化学和蛋白免疫印迹检测紧密连接蛋白claudin-1、occludin和ZO-1肠黏膜的定位和表达。结果紧密连接蛋白claudin-1、occludin和ZO-1在PD大鼠模型十二指肠黏膜上均有表达,但仅ZO-1(P<0.001)和occludin(P<0.01)表达明显下调,而claudin-1无显著变化。结论 PD大鼠模型十二指肠黏膜紧密连接蛋白ZO-1、occludin的表达显著下调,可能与帕金森病十二指肠溃疡的发生发展相关。
Objective To investigate the alterations of tight junction proteins in duodenal mucosa from 6-hydroxydopamine（ 6-OHDA）-induced Parkinson＇s disease（ PD） model. Methods Rats were subjected with 6-OHDA in bilateral substantia nigra（ SN） as PD model. The location and quantitative detecting techniques including immunohisto-chemistry and western blotting were used to determine the expressions and alterations of tight junction proteins（ claudin-1,occludin,ZO-1）. Results Claudin-1,occludin and ZO-1 were highly expressed in the duodenal mucosa of PD models,where the expressions of occluding（ P〈0. 001） and ZO-1（ P 0. 001）were much lower,while the expression of claudin-1 had no alteration. Conclusions The down-regulated expression of occludin and ZO-1 was detected in duodenal mucosa of PD models,which might be related to duodenal ulcer in PD.
作者
张冬妮
王忆安
李广文
闫力铭
冯小燕
朱进霞
ZHANG Dong-ni WANG Yi-an LI Guang-wen YAN Li-ming FENG Xiao-yan ZHU Jin-xia(Dept. of Physiology and Pathophysiology Grade 2013 Clinical Medicine Grade 2012 Basic Medicine, School of Basic Medical Science, Capital Medical University, Beijing 100069, China)
出处
《基础医学与临床》
CSCD
2017年第10期1359-1362,共4页
Basic and Clinical Medicine
基金
国家自然科学基金(31500937)
本科生科研创新项目(XSKY2016014)
