期刊文献+

不同氧浓度培养对体外受精-胚胎移植及子代出生缺陷的影响 被引量:5

Effect of oxygen concentration on outcome of in-vitro fertilization-embryo transfer
下载PDF
导出
摘要 目的:比较低氧浓度与高氧浓度培养对体外受精—胚胎移植中胚胎发育、妊娠结局以及子代出生缺陷的影响。方法:根据胚胎体外培养的氧浓度,将2013至2015年在浙江大学医学院附属妇产科医院行体外受精—胚胎移植的患者随机分为低氧浓度组(n=2036,培养体系含氧气体积分数为5%)和高氧浓度组(n=4617,培养体系含氧气体积分数为20%)。比较两组间受精率、优质胚胎率、临床妊娠率、异位妊娠率、流产率以及子代出生缺陷率等结果指标。结果:低氧浓度组优质胚胎率高于高氧浓度组(P<0.05),但两组间受精率、临床妊娠率、异位妊娠率、流产率和子代出生缺陷的发生率差异均无统计学意义(均P>0.05)。结论:体外受精—胚胎移植采用低氧环境培养可能有助于提高胚胎发育潜能,但对妊娠结局和子代出生缺陷的影响不明显。 Objective:To investigate the effect of low and high oxygen concentration on embryo development, pregnancy outcome and birth defects of in vitro fertilization-embryo transfer (IVF-ET). Methods:According to the oxygen concentration of in vitro culture environment, the IVF-ET performed in the Women's Hospital, Zhejiang University School of Medicine during 2013 and 2015 were divided into low oxygen concentration group (n=2036, 5% O2) and high oxygen concentration group (n=4617, 20% O2). The rate of fertilization, good quality embryo, clinical pregnancy, ectopic pregnancy, abortion and birth defect were compared between two groups. Results:The good quality embryo rate was significantly higher in the low oxygen concentration group (P〈0.05). However, no significant differences were found between two groups in the rate of fertilization, clinical pregnancy, ectopic pregnancy, abortion and birth defect (all P〉0.05). Conclusion:Low oxygen environment may improve the potential of embryonic development, but its impact on pregnancy outcome and birth defect is not significant.
出处 《浙江大学学报(医学版)》 CAS CSCD 北大核心 2017年第3期290-294,共5页 Journal of Zhejiang University(Medical Sciences)
基金 国家自然科学基金(81370761) 浙江省医药卫生科技计划(2014KYA252 201493615)
关键词 受精 体外 胚胎移植 新生儿筛查 先天畸形 胚胎发育 妊娠 结局 Oxygen Fertilization in vitro Embryo transfer Neonatal screening Congenital abnormalities Embryonic development Pregnancy outcome
  • 相关文献

参考文献3

二级参考文献65

  • 1Fischer B, Bavister BD. Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits [J]. J Reprod Fertil, 1993, 99(2): 673-679.
  • 2Ottosen LD, Hindkaer J, Husth M, et al. Observations on intrauterine oxygen tension measured by fibre-optic microsensors [J]. Reprod Biomed Online, 2006, 13 (3): 380-385.
  • 3Kovacic B, Sajko MC, Vlaisavljevi6 V. A prospective, randomized trial on the effect of atmospheric versus reduced oxygen concentration on the outcome ofintracytoplasmic sperm injection cycles[J]. Fertil Steril, 2010, 94(2): 511-519.
  • 4Pacella L, Zander-Fox DL, Armstrong DT, et al. Armstronget al. Women with reduced ovarian reserve or advanced maternal age have an altered follicular environment[J]. Fertil Steril, 2012, 98(4): 986-994.
  • 5Hu Y, Maxson WS, Hoffman DI, et al. Hoffman et al. Maximizing pregnancy rates and limiting higher-order multiple conceptions by determining the optimal number of embryos to transfer based on quality [ J ]. Fertil Steril, 1998, 69(4): 650-657.
  • 6Kovacic B, Vlaisavljevi6 V. Influence of atmospheric versus reduced oxygen concentration on development of human blastocysts in vitro: a prospective study on sibling oocytes [J]. Reprod Biomed Online, 2008, 17 (2) : 229-236.
  • 7Rho GJ, S B, Kim DS, Son WJ,et al. Influence of in vitro oxygen concentrations on preimplantation embryo development, gene expression and production of Hanwoo calves following embryo transfer [J]. Mol Reprod Dev, 2007, 74(4): 486-496.
  • 8Petersen A, Mikkelsen AL, Lindenberg S. The impact of oxygen tension on developmental competence of post- thaw human embryos [J]. Acta Obstet Gynecol Scand, 2005, 84(12): 1181-1184.
  • 9Dumoulin JC, Meijers C J, Bras M, et al. Effect of oxygen concentration on human in-vitro fertilization and embryo culture[J]. Hum Reprod, 1999, 14(2): 465- 469.
  • 10Kea B, Gebhardt J, Watt J, et al. Effect of reduced oxygen concentrations on the outcome of in vitro fertilization[J]. Fertil Steril, 2007, 87(1): 213-216.

共引文献5

同被引文献50

引证文献5

二级引证文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部