摘要
目的采用UPLC-MS/MS法探讨甘草与麻黄配伍后对甘草中入血成分甘草酸、甘草次酸和甘草苷药动学的影响。方法将12只大鼠随机分成2组,各组分别灌胃给予单味甘草和麻黄-甘草(2∶1)水煎液后,于不同时间点采血,所得血药浓度-时间数据经DAS3.2.2计算相关的药动学参数。结果与单味甘草组相比,麻黄-甘草(2∶1)组甘草苷、甘草次酸和甘草酸的AUC_(0-t)(药-时曲线下面积)和C_(max)(最大血药浓度)均下降,MRT_(0-t)(平均驻留时间)和t_(1/2z)(血浆消除半衰期)均推迟,V_z/F(表观分布容积/吸收分数)均增大;甘草苷和甘草酸的CL_z/F(总体清除率/吸收分数)均增大;甘草酸的T_(max)(达峰时间)缩短了,甘草次酸的T_(max)则推迟了。结论甘草与麻黄配伍后,降低了甘草酸、甘草次酸和甘草苷的生物利用度,延缓了三者在体内的消除,增大了三者在体内的分布体积。
AIM To investigate the effects of the compatibility of Ephedrae Herba （Mahuang） and Glycyrrhizae Radix et Rhizoma （Gancao） on pharmacokinetic changes of the plasma levels of glycyrrhizic acid,glycyrrhetinic acid and liquiritin by UPLC-MS/MS.METHODS Twelve SD rats were randomly divided into two groups and orally administered with Glycyrrhizae Radix et Rhizoma and Ephedrae Herba-Glycyrrhizae Radix et Rhizoma （2 ∶ 1）Decoction.Blood samples were collected at different time points,and the main pharmacokinetic parameters were calculated by DAS3.2.2 software.RESULTS Compared with Glycyrrhizae Radix et Rhizoma group,the AUC（0-t） and Cmaxwere decreased,the MRT（0-t）and t（1/2z） were extended,the Vz/F was increased for glycyrrhizic acid,glycyrrhetinic acid and liquiritin; the CLz/F was increased for glycyrrhizic acid and liquiritin; the Tmaxof glycyrrhizic acid was shortened and the Tmaxof glycyrrhetinic acid was extended.CONCLUSION After combining Glycyrrhizae Radix et Rhizoma and Ephedrae Herba,the bioavailability of glycyrrhizic acid,glycyrrhetinic acid and liquiritin are reduced,the elimination of them delays,and the distribution volume increases.
出处
《中成药》
CAS
CSCD
北大核心
2017年第10期2009-2014,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金重点项目(81030066)
深圳市坪山新区卫生系统科研资助项目(201538)
关键词
麻黄
甘草
药对
甘草酸
甘草次酸
甘草苷
药动学
Ephedrae Herba （Mahuang）
Glycyrrhizae Radix et Rhizoma （Gancao）
herbal pair
glycyrrhiz-ic acid
glycyrrhetinic acid
liquiritin
pharmacokinetics
