摘要
建立了高效液相色谱串联质谱仪对酒类产品中11种PDE-5抑制剂及其衍生剂的快速分析方法。样品经乙腈∶水(1∶1,v/v)超声提取,Atlantis d C_(18)色谱柱(2.1×150 mm,3μm)为分析柱,0.1%甲酸溶液和0.1%甲酸乙腈为流动相,电喷雾正离子多反应监测模式进行检测,外标法定量。试验结果表明,该方法的RSD值范围为0.57%~11.69%,回收率范围在60.0%~118.6%,该方法的线性相关性良好,检测低限、精密度和准确度均符合要求,能够满足酒类产品中11种PDE-5抑制剂及其衍生剂的检测要求。
A method of high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） had been developed for the determination of 11 kinds of PDE-5 inhibitors and their derivatives in alcoholic drinks. Samples were diluted with acetonitrile： water （1 ： 1, v：v） and the analyte was separated by art Atlantis dC 18 cohmm （2.1 × 150 ram, 3 μm）using a mobile phase consisting of 0.1% formic acid and acetonitrile with 0.1% formic acid. The analyte was detected in multiple-reaction monitoring （MRM） mode via posi- tive electrospray ionization （ESI＋）. The determination results showed that the RSD was between 0.57 % to 11.69 % and the recovery was between 60.0 % to 118.6 % at three spiked levels. The method was characterized by its excellent purification effect, high sensitiv- ity and good accuracy, and it could be used for the determination of 11 kinds of PDE-5 inhibitors and their derivatives in alcoholic drinks.
作者
翟纹静
ZHAI Wenjing(Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou, Jiangsu 215100 Suzhou World Standard Testing Technology Co. Ltd., Suzhou, Jiangsu 215100, China)
出处
《酿酒科技》
2017年第10期126-129,共4页
Liquor-Making Science & Technology
关键词
液质联用仪
酒类
PDE-5抑制剂
high performance liquid chromatography tandem mass spectrometry （LC-MS/MS）
alcoholic drinks
PDE-5 inhibitor
