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荧光定量PCR法与ELISA法联合检测乙型肝炎的临床价值探讨 被引量:18

The study to the clinical value of fluorescence quantitative PCR method and ELISA method combined detection of hepatitis B
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摘要 目的研究荧光定量PCR法(FQ-PCR)检测乙肝病毒DNA(HBV-DNA)与ELISA法检测乙肝免疫学标志物(HBV-M)的临床价值。方法本研究选取2016年1月~2017年6月在西安高新医院检测的382例乙肝患者为研究对象,全部患者均采用荧光定量PCR法和ELISA法分别对HBV-DNA和乙肝两对半等免疫学指标进行检测,免疫学指标的检测顺序为乙型肝炎病毒表面抗原(HBs Ag)、乙肝病毒表面抗体(HBs Ab)、乙肝病毒e抗原(HBe Ag)、乙肝病毒e抗体(HBe Ab)、乙肝病毒核心抗体(HBc Ab)。比较不同HBV-M模式检测结果。结果模式1(大三阳组)HBV DNA阳性率最高,为94.6%,显著高于其他组,差异具有统计学意义(P<0.05)。模式1(大三阳组)的HBV DNA表达水平为(6.57±1.51)lg copies/ml,明显高于其他模式的HBV DNA表达水平,差异具有统计学意义(P<0.05)。结论采用FQ-PCR和HBV免疫学标志物检测相结合的方法,对临床乙型肝炎患者的早期诊断、病情判断、治疗方案的选择及预后具有重要指导价值。 Objective:To investigate the clinical value of fluorescence quantitative PCR in detection of HBV DNA and ELISA in detection of HBV immunological markers. Methods:382 cases of hepatitis B patients in Xi an gao xin hospital were selected as the object of study from January 2016 to June 2017. The HBV-DNA and HBV immunological markers of all patients were detected by fluorescence quantitative PCR and ELISA respectively. The order of HBV immunological markers:Hepatitis B surface Antigen(HBs Ag),Hepatitis B surface Antibody(HBs Ag),Hepatitis B e Antigen(HBe Ag),Hepatitis B e Antibody(HBeAb),Hepatitis B core Antibody(HBc Ab).Different HBV-M model test results were compared. Results:HBV-DNA positive rate of Pattern 1(HBe Ag positive patients)was 94.6%,which was significantly higher than that in other pattern and the difference was statistically significant(P〈 0.05). HBV-DNA expression level of Pattern 1(HBe Ag positive patients)was which was(6.57±1.51)lg copies/ml,significantly higher than that in other pattern and the difference was statistically significant(P〈 0.05). Conclusion:The combination of FQ-PCR and HBV immunological markers detection has an important guiding value for early diagnosis,treatment and prognosis of patients with hepatitis B.
作者 高娟 刘静
机构地区 西安高新医院
出处 《中国优生与遗传杂志》 2017年第9期29-31,共3页 Chinese Journal of Birth Health & Heredity
关键词 荧光定量PCR HBV-DNA 乙肝免疫学标志物 酶联免疫吸附实验 FQ-PCR HBV-DNA Hepatitis B immunological markers ELISA
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