摘要
目的研究鼻息肉的全基因表达谱情况,探寻其与正常鼻腔黏膜的基因表达差异。方法提取鼻息肉中的RNA,将RNA合成双链的c DNA,将标记的c DNA在Roche Nimble Gen基因芯片上进行杂交。应用Axon Gene Pix 4000B scanner进行扫描,应用Nimble Scan软件进行图像分析,探针及基因水平标准化后进行计算。结果与正常鼻腔黏膜相比,鼻息肉中有1 000个基因表达上调,占2.22%(1 000/45 033),有1 123个基因表达下调,占2.49%(1 123/45 033)。经过信号通路分析,发现在核糖体、蛋白酶体、柠檬酸循环、膀胱癌、非小细胞肺癌、神经胶质瘤、子宫内膜癌、内质网蛋白合成、慢性髓样白血病、谷胱甘肽代谢等信号通路中相关基因表达上调,在嗅觉传导、自然杀伤细胞介导的细胞毒性、细胞色素P450的化合物代谢、细胞色素P450的药物代谢、抗原加工和呈递、疟疾、移植物抗宿主疾病、视黄醇代谢、亚油酸代谢、戊糖和葡萄糖醛酸转换等信号通路中相关基因表达下调。结论鼻息肉的发生、发展与多个基因及信号通路的表达升高或降低有关,鼻息肉全基因表达谱的研究为探求鼻息肉的发生机制提供了有益的线索。
Objective To analyze the gene expression profiles of nasal polyp and gene expression differences between the nasal pol- yp and normal nasal mucosa. Methods Total RNA from nasal polyp tissues was purified and synthesized into double-stranded cDNA. The cDNA was labeled and hybridized in a NimbleGen hybridization chamber. The slides were scanned using the Axon GenePix 4000B microarray scanner. Scanned images were analyzed using NimbleScan software. The probes and gene levels were standardized and calcu- lated. Results Compared with normal nasal mucosa tissues, expression of 2.22% （ 1 000/45 033） of genes was up-regulated in all cases of nasal polyp tissues, while 2.49% （1 123/45 033） of genes were down-regulated in all cases of nasal polyp tissues. We found genes related to the ribosome, proteasome, citrate cycle （TCA cycle）, bladder cancer, non-small cell lung cancer, glioma, endometrial cancer, protein processing in the endoplasmic reticulum, chronic myeloid leukemia, and glutathione metabolism were up-regulated.Genes related to olfac- tory transduction, natural killer cell-mediated cytotoxicity, metabolism of xenobiotics by cytoehrome P450, drug metabolism-cytochrome P450, antigen processing and presentation, malaria, graft-versus-host disease, retinol metabolism, linoleic acid metabolism, and pentose and glucuronate intereonversions were down-regulated. Conclusion Multiple genes or pathways may be involved in the occurrence and development of nasal polyp.~Gene expression profiling provides insight into the mechanism of nasal polyp development.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2017年第10期895-898,共4页
Journal of China Medical University
基金
辽宁省科学事业公益研究基金(2013001010)
沈阳市科学技术计划(F11-264-1-07
F13-220-9-21)
关键词
鼻息肉
基因芯片
基因表达
信号通路
nasal polyp
gene chip
gene expression
signaling pathway
