摘要
将壳聚糖缓释微球与可降解多孔支架复合,构建可次第释放不同生长因子的骨组织工程支架,并进行表征和性能研究。首先,制备载骨形态发生蛋白-2的壳聚糖微球(BMP-2-CMs),然后将微球与纳米羟基磷灰石/羟基乙酸(nHA/PLGA)及血小板衍生生长因子(PDGF)按照一定的比例混合,通过粒子沥虑-冷冻干燥复合工艺制备PDGF/BMP-2-CMs/nHA/PLGA复合支架。BMP-2-CMs呈规则球形,粒径分布在4~10μm之间,BMP-2包封率为65.9%,载药量为0.134%。PDGF/BMP-2-CMs/nHA/PLGA复合支架孔径为100~200μm,孔隙率为51.2%,抗压强度为7.7MPa,8周降解率为20.1%。7d时,PDGF和BMP-2累计释放率分别为75.0%和42.2%;14d时,PDGF和BMP-2累计释放率分别为79.9%和53.5%。分析可知,复合支架中释放的PDGF和BMP-2能够持续有效地促进人脐静脉血管内皮细胞(HUVEC)和成骨细胞(MG63)的增殖和分化,具有良好的生物活性。由此可得,PDGF/BMP-2-CMs/nHA/PLGA复合支架能够次第释放PDGF和BMP-2,且能够显著促进HUVEC和MG63的增殖和分化。
In order to construct a bone regeneration scaffold that could sequentially releasedifferent growth factorsat proper stage, the composite of sustained-release microspheres and biodegradable porous scaffolds was prepared in this study. Firstly, BMP-2 loaded chitosan microspheres （CMs） were prepared and compounded to the nano hydroxyapatite/poly lactic-co-glycolic acid （nHA/PLGA） with PDGF to build a scaffold that can re lease growth factors sequentially. The results showed that the BMP-2-CMs were spherical shape with diameters of ,1-10 μm. The encapsulation efficiency of the BMP-2 in CMs was 65.9% and the loading capacity was 0.134%. The prepared PDGF/BMP-2-CMs/nHA/PI.GA scaffold possessed 100-200 μm pore diameter, 51.2% porosity, 7.7 MPa compressive strength and 20.1% degradation at 8 weeks. The cumulative releases of PDGF and BMP-2 from PDGF/BMP-2-CMs/nHA/PLGA scaffolds were respectively about 75.00//oo and 42.2% at 7 d and 79.9% and 53.5% at 14 d. PDGF and BMP-2 released from scaffold could promote cell proliferation and differentiation effectively. The biological activity of these growth factors was more durable and effective. The results demonstrated that PDGF/BMP-2-CMs/nHA/PLGA scaffolds had excellent drug sustained-release performance, which would controllably release PD（;F and BMP-2 sequentially and maintain their biological activity effectively.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2017年第10期10159-10164,共6页
Journal of Functional Materials
基金
国家自然科学基金资助项目(81401535)
重庆市教委科技资助项目(KJ1702035)
福建省生物化工技术重点实验室开放研究课题资助项目(Z17X0003)
关键词
壳聚糖
羟基乙酸
复合支架
次第释放
增殖分化
chitosan
poly lactic-co-glycolic acid
composite scaffolds
sequential release
proliferation and differentiation
