摘要
目的探讨人参皂苷Ro对胃癌细胞MFC增殖和凋亡的影响。方法采用MTT染色法检测MFC细胞的增殖抑制率;流式细胞仪检测MFC细胞周期和凋亡情况;MFC移植瘤小鼠,经受试药物处理后,剥取瘤组织称量并计算抑瘤率。结果与对照组比较,人参皂苷Ro在6~96μg·m L^(-1)范围内对MFC细胞增殖的抑制率随浓度的增加而显著升高。与对照组比较,人参皂苷Ro高剂量组对MFC细胞G0/G1期和凋亡率明显升高,G2/M期,S期明显降低;人参皂苷Ro各剂量组均能显著抑制肿瘤生长,与模型组比较,各给药组肿瘤的质量明显降低,其中,高剂量组的抑瘤率为62.7%。结论人参皂苷Ro对MFC移植瘤具有抑制生长的作用,这可能与诱导MFC细胞凋亡和抑制增殖有关。
OBJECTIVE To investigate the antitumor effects of ginsengside Ro on proliferation and apoptosis of murine gastric cancer MFC cells. METHODS MTT was employed to detect proliferation of MFC cells. Flow cytometry was used to examine the cell cycle progression and apoptosis of MFC cells. MFC tumor bearing mice were treated,and the tumor tissue was dissected and weighted. The tumor inhibition rate was calculated. RESULTS Ginsengside Ro was within 6-96 μg·m L^（-1）. MFC cell proliferation inhibition rate was significantly increased with the increase of the concentration（ compared with that in the 6. 0 μg·m L^（-1）group）. The ratio of G0/G1 cells was significantly increased,and the ratio of G2/M cells was significantly reduced in high-dose ginsengside Ro groups as compared with the blank group. All doses of ginsengside Ro significantly inhibited the tumor growth（ compared with that in the model group）,and the tumor inhibition rate in high-dose ginsengside Ro was 62. 7%. CONCLUSION Ginsengside Ro has antitumor effect on tumor bearing mice. The mechanism may be related to the inhibition of cell proliferation and the induction of cell apoptosis.
出处
《华西药学杂志》
CAS
CSCD
2017年第5期499-501,共3页
West China Journal of Pharmaceutical Sciences
基金
陕西省科技厅资助项目(2012KTCL03-14)
陕西省科技厅中药现代化项目[2009K19-03(2)]
