摘要
目的:探讨M2型肿瘤相关巨噬细胞(M2型TAM)浸润与食管鳞癌发生、浸润、转移的关系及其可能机制。方法:采用免疫组化SP法分别检测50例食管鳞癌和50例正常食管黏膜组织中CD206(M2型TAM特异性标志物)、IL-8、MCP-1蛋白的表达;应用原位杂交方法分别检测上述组织中IL-8、MCP-1 mRNA的表达。结果:食管鳞癌组织中M2型TAM的浸润数量多于正常食管黏膜(P=0.032);食管鳞癌间质中IL-8、MCP-1蛋白及mRNA阳性表达率高于正常食管黏膜组织(P<0.05);M2型TAM浸润数量与食管鳞癌的浸润深度、淋巴结转移及患者的TNM分期有关(P<0.05),与患者的年龄、性别无关(P>0.05);食管鳞癌间质中M2型TAM的浸润数量与IL-8、MCP-1蛋白和mRNA的表达呈正关联(蛋白:rP=0.432、0.315;mRNA:rP=0.373、0.332,P均<0.05)。结论:M2型TAM与食管鳞癌的发生、浸润、转移有关,其机制可能与TAM分泌的趋化因子IL-8和MCP-1有关。
Aim： To investigate the relationship between the infiltration of M2 type tumor associated macrophages（ M2 type TAM） and the occurrence,invasion and metastasis of esophageal squamous cell carcinoma（ ESCC） and its possible mechanism. Methods： The expressions of CD206,IL-8,MCP-1 proteins and IL-8,MCP-1 mRNA were detected using immunohistochemical method and in situ hybridization in 50 cases of the interstitial of ESCC and 50 cases of normal esophageal mucosa. Results： The number of M2 type TAM in the interstitial of ESCC was significantly higher than that in normal esophageal mucosa tissue（ P = 0. 032）; the positive expression rates of IL-8,MCP-1 protein and mRNA in the interstitial tissue of ESCC were significantly higher than those in the interstitial tissue of normal esophageal mucosa（ P〈0. 05）. The number of M2 type TAM was significantly associated with the depth of tumor invasion,lymph node metastasis and TNM stage（ P〈0. 05）,but not with the patients＇ age or gender（ P〉0. 05）. The number of M2 type TAM in ESCC was positively correlated with the expressions of IL-8,MCP-1 protein（ rP= 0. 432,0. 315） and mRNA（ rP= 0. 373,0. 332,P〈0. 05）.Conclusion： M2 type TAM may be related with the occurrence,invasion and metastasis of ESCC,and the mechanism may be related with chemotactic factor IL-8 and MCP-1 secreted by M2 type TAM.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2017年第5期527-531,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省重点科技攻关计划项目132102310086
河南省基础与前沿技术研究计划项目142300410076
国家自然科学基金81272370
