摘要
目的探讨茶多酚对吗啡所致便秘的预防作用及其作用机制。方法雌性昆明种小鼠按随机数字表法分为空白对照组、模型对照组、茶多酚组、茶多酚+吗啡组,每组10只。茶多酚组和茶多酚+吗啡组小鼠灌胃给予茶多酚100 mg·kg^(-1),空白对照组和模型对照组给予0.5%羧甲基纤维素钠溶液0.1 m L·kg^(-1),连续4 d。给药第4天,模型对照组和茶多酚+吗啡组小鼠腹腔注射吗啡20 mg·kg^(-1),茶多酚组和空白对照组腹腔注射0.9%氯化钠溶液0.1 m L·kg^(-1),15 min后小鼠灌胃给予5%墨汁液0.2 m L。热板法检测各组小鼠的第1次舔足时间,观察茶多酚对吗啡镇痛效果的影响;酶联免疫吸附测定(ELISA)法检测各组小鼠小肠内胃动素、P-物质及生长抑素的含量。结果与空白对照组比较,模型对照组墨汁推进长度和墨汁推进率明显降低(P<0.01);茶多酚组墨汁液推进长度和墨汁推进率显著增加(P<0.05)。与模型对照组比较,茶多酚+吗啡组墨汁推进长度和墨汁推进率显著升高(P<0.01)。空白对照组和茶多酚组小鼠第1次舔足时间分别为(8.64±2.72),(9.11±2.13)s,模型对照组和茶多酚+吗啡组小鼠第1次舔足时间分别为(18.79±3.58),(20.10±3.72)s。与空白对照组比较,模型对照组胃动素、P物质含量减少(P<0.05),茶多酚组胃动素、P物质明显增加(P<0.05);与模型对照组比较,茶多酚+吗啡组胃动素、P物质含量明显增加(P<0.05)。与空白对照组比较,模型对照组生长抑素含量增加,而茶多酚组和茶多酚+吗啡组生长抑素含量显著减少(P<0.05)。结论茶多酚在不降低疼痛治疗效果的前提下,能有效改善小鼠肠蠕动预防吗啡所致便秘,这一作用与茶多酚对肠道胃动素、P物质和生长抑素的含量调节有关。
Objective To explore the preventive effect and mechanism of tea polyphenols on morphine-induced constipation. Methods Female Kunming mice were randomly divided into 4 groups （ 10 mice per group） , including blank control group, model control group, tea polyphenols group and tea polyphenols＋morphine group. Tea polyphenols group and tea polyphenols ＋ morphine group were pretreated with 100 mg·kg^-1 of tea polyphenols for 4 days, meanwhile blank control group and model control group were preteated with 0.1 mL·kg^-1 of 0.5% CMC-Na for 4 days. On the fourth day model control group and tea polyphenols ＋ morphine group were intraperitoneal injected 20 mg·kg^-1 morphine, otherwise blank control group and tea polyphenols group were injected with 0.1 mL·kg^-1 of 0.9% sodium chloride solution. Then mice were given 0.2 mL of 5% inksolution by intragastric administration 15 min later. The latency to paw licking was detected in hot plate test to evaluate the effect of tea polyphenols on morphine analgesia. The levele of motilin （MLT）, substance P （SP） and somatostatin （SS） in intestinal tissue were determined by enzyme-linked immunosorbent assay （ ELISA） among groups. Results Compared with the blank control group, the length of propelling ink and the propelling rate of ink were significantly lower in the model control group （ P〈0.01） , meanwhile tea polyphenols group were much higher （ P〈0. 05 ） . Compared with model control group, the length of propelling ink and the propelling rate of ink were significantly higher in tea polyphenols ＋ morphine group mice （ P〈0.05） . The first paw licking time of control group and tea polyphenols group were （8.64 ＋ 2.72）s and （9.11 ＋ 2.13） s, and the time of model control group and tea polyphenols ＋ morphine group were （18.79±3.58）s and （20.10±3.72） s. The contents of MLT and SP were reduced in model control group （P〈0.05）, but significantly increased in tea polyphenols group （P〈0.05） compared with blank control group. Compared with the model control group, MLT and SP had an obviously increase in tea polyphenols ＋morphine group （P〈0.05）. Compared with blank control group, the content of SS was increased in model control group, but decreased markedly in tea polyphenols group and tea polyphenols＋morphine group （ P〈0.05） . Conclusion Tea polyphenols can prevent the morphine-induced constipation without decreasing the analgesic effect of morphine, which is related to the regulation of the content of MLT, SP and SS.
出处
《医药导报》
CAS
2017年第10期1129-1132,共4页
Herald of Medicine
关键词
茶多酚
吗啡
便秘
镇痛
Tea polyphenols
Morphine
Constipation
Analgesia
