摘要
【目的】从盐生植物盐角草(Salicornia europaea)中克隆得到一个参与光合反应光系统I(PSI)复合蛋白H亚基的Psa H基因,分析该基因进行生物信息学,为研究该基因的作用奠定基础并为改良作物耐盐性分子育种提供候选基因。【方法】以盐生植物盐角草为实验材料,采用RT-PCR方法克隆Psa H基因,利用NCBI、MEGA、Expasy等生物信息学工具对其核酸序列、编码的氨基酸序列及蛋白质的结构和功能进行分析。【结果】克隆得到一个与耐盐有关的基因,命名为Se Psa H,属于光系统I(PSI)家族H亚基成员,其开放阅读框(ORF)为438 bp,基因编码145个氨基酸,预测蛋白分子量为15.3 k D,等电点9.84,是亲水性蛋白;系统进化树分析表明其与菠菜亲缘关系较近,通过对该蛋白保守性分析发现其含有4个保守性结构域。【结论】获得盐角草Se Psa H基因,为进一步研究该基因耐盐功能及其在盐生植物耐盐机制中的作用提供了基础。
【Objective】To clone a novel Psa H gene from Salicornia europaea,and analyze its biological information for better understanding its role in function of salt-tolerance.【Method】Salicornia europaea was used as the plant material to clone the full-length c DNA sequence of Se Psa H by RT-PCR. The encoding region and amino acid sequence of Psa H gene,and the structure and function of protein encoded by protein were analyzed by NCBI,MEGA and Expasy and other online Bioinformatics bioinformatics software for Se Psa H gene.【Result】Full-length c DNA sequence encoding photosystem I reaction center subunit H was cloned from Salicornia europaea and designated by the name of Se Psa H,which was an opening reading frame of 438 bp encoding 145 amino acids. The putative protein molecular weight was 15. 3 k D and its theoretical isoelectric points was 9. 84,Se Psa H was a hydrophilic protein; Phylogenetic analysis showed that Se Psa H gene and Spinacia oleracea were closely related; Through the conservative analysis of the protein,we found that there were4 conserved domain structures.【Conclusion】Se Psa H gene was cloned,which has laid the foundation for further study on the gene function and the role in salt-tolerance of Salicornia europaea.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2017年第9期1613-1620,共8页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区自然科学基金项目"碱蓬SsPSI基因提高转基因烟草耐旱性的研究"(2015211A028)
新疆农业科学院优秀青年科技人才基金"盐生植物盐角草耐盐基因的发掘与功能研究"(xjnky-2012-003)~~
关键词
盐角草
SePsaH基因
生物信息
Salicornia europaea
photosystem I reaction center subunit H
bioinformatics
