摘要
目的:分析采用放疗与中药"君-佐"相伍的理论对肝癌细胞放疗增敏效果,为临床应用提供依据。方法:将HepG2细胞分为5组,检测各组细胞克隆形成数及克隆形成率,检测各组细胞凋亡率,并分析HIF-1α、CA-9、VEGF或β-actin的蛋白及mRNA水平。结果:药物加放疗组HepG2细胞克隆形成数及克隆形成率显著低于其他组(P<0.05);药物加放疗组HepG2细胞凋亡率明显高于其他组,且差异存在统计学意义(P<0.05);药物加放疗组HepG2细胞HIF-1α/β-actin及VEGF/β-actin蛋白及mRNA水平显著低于其他组,但药物加放疗组HepG2细胞CA-9/β-actin蛋白及mRNA水平显著高于其他组。结论:采用放疗与中药"君-佐"相伍的理论对肝癌细胞处理后,可能提高细胞凋亡率及放疗增敏效果。
Objective:Analyze the sensitization effect of radiotherapy and traditional Chinese medicine 'Jun Zuo'theory on liver cancer cells,and to provide basis for clinical application.Methods:HepG2 cells were divided into five group were assayed by colony formation and clone formation rate,apoptosis rate was detected,and the analysis of protein and mRNA levels of HIF-1,CA-9,VEGF alpha or beta-actin.Results:The drug plus radiotherapy group HepG2 cell colony formation and clone formation rate was significantly lower than that of other groups(P<0.05);drug plus radiotherapy group the apoptosis rate of HepG2 cells was significantly higher than other groups,and the difference was statistical significance(P<0.05);drug plus radiotherapy group HepG2 cells HIF-1 alpha beta-actin and VEGF/beta/-actin protein and mRNA levels were significantly lower than other groups,but the content of HepG2 cells plus radiotherapy group CA-9/beta-actin protein and mRNA levels were significantly higher than that in other groups.Conclusions:The use of radiotherapy and Chinese medicine'Jun Zuo'theory for treating liver cells,can effectively reduce the dry cell,improve the rate of cell apoptosis,and the expression level through the regulation of HIF-1 alpha and VEGF and CA-9,to improve the radiation sensitizing effect of hepatocellular carcinoma cells.
出处
《陕西中医》
2017年第11期1610-1612,共3页
Shaanxi Journal of Traditional Chinese Medicine
基金
陕西省科学技术研究发展计划项目(2013KW-26-03)
陕西省中医药管理局项目(JC38)
关键词
肝肿瘤/中医药疗法
放射疗法
@中药“君-佐”相伍的理论
辐射增敏药
Liver neoplasms/traditional
Chinese medicine therapy
Radiotherapy
@Traditional
Chinese medicine'Jun Zuo'
Radiation-sensitizing agents
