摘要
为了研究血根碱对蛋鸡输卵管炎的治疗作用及其机理,将某鸡场自然发病的输卵管炎病鸡50只随机分成5组,Ⅰ、Ⅱ、Ⅲ组为试验组,分别用高、中、低剂量的血根碱治疗,Ⅳ组西药对照组,用阿莫西林治疗,Ⅴ组为空白组,不治疗,另取10只健康鸡为Ⅵ组,即健康对照组。观察各组的临床表现,并于用药后第8天采血测定血清中IL-2含量,取输卵管组织制作切片观察,并测定IL-2的基因表达水平。结果显示:试验组蛋鸡临床及镜检炎症表现轻微,血清中IL-2含量较空白组低,且IL-2的基因表达量也较空白组低,尤以Ⅲ组的疗效最佳。提示血根碱对蛋鸡输卵管炎有一定的治疗作用,低剂量疗效最佳,其机理可能是通过下调IL-2的表达来实现其抗炎作用。
In order to study the therapeutic effect and mechanism of sanguinarine on layers＇ salpingitis,50 layers naturally infected with salpingitis in a chicken farm were randomly divided into 5 groups. Group Ⅰ,Ⅱ and Ⅲ were the trial groups,treated with high,medium and low dose of sanguinarine respectively,group Ⅳ was the western drug control group treated with amoxicillin,group Ⅴ was the control group without treatment. 10 healthy layers were taken to be group Ⅵ,being healthy control group. The clinical manifestations of each group were observed,and the blood samples were collected to determine the content of IL-2 in serum on 8 th day,and the oviducts of 3 layers in each group were taken to be tissue slices to observe the pathologicalchanges,and the gene expression of IL-2 was determined. The results showed that the inflammation reactions in trial groups were slighter in visual and microscope observation,the IL-2 contents in serum of the trial groups were lower than those of the control group,and the gene expressions of IL-2 were also lower than those of the control group,above of all,the therapeutic effect in group Ⅲ was the best. It is indicated that sanguinarine present good therapeutic effect on layers＇ salpingitis,especially the low dose,the therapeutic effect mechanism may achieve through the down-regulation of IL-2 gene expression.
作者
姬晓琪
钟杰
张明军
梁丰达
袁景阳
刘兆颖
曾建国
JI Xiaoqi ZHONG Jie ZHANG Mingjun LIANG Fengda YUAN Jingyang LIU Zhaoying ZENG Jianguo(Hunan Agricultural University, Changsha Hunan 410128, China Animal Epidemic Prevention and Quarantine Station in Zhentou Town of Liuyang City, Liuyang Hunan 410319, China)
出处
《中兽医医药杂志》
2017年第5期59-62,共4页
Journal of Traditional Chinese Veterinary Medicine
基金
湖南省自然科学基金(项目编号:2016JJ4040)
