摘要
目的探讨腹腔注射给药Ghrelin对小鼠海马神经再生的影响。方法本研究将小鼠随机分为Ghrelin组及对照组,两组均腹腔注射相同剂量Brd U(100 mg/kg,早晚各1次,连续5天),目的用来标记海马齿状回颗粒细胞下区(Subgranular Zone,SGZ)增生的细胞。处理组小鼠给予腹腔注射Ghrelin(80μg/kg,一天一次,连续8 d),而对照组注射等量的生理盐水。1周后进行免疫组化分析。结果 BrdU免疫阳性(BrdU+)细胞在海马齿状回中广泛表达;Ghrelin处理组BrdU+细胞数显著多于生理盐水对照组(t=3.43,P<0.01)。结论腹腔注射Ghrelin(80μg/kg)促进海马齿状回神经再生。
Objective： To explore the effect of intraperitoneal injection of Ghrelin on hippocampus neurogenesis. Methods： Mice were randomly divided into Ghrelin group and control group. Both groups were given intraperitoneal injection of equal BrdU（ 100 mg/kg,twice a day,continuous injection of 5 days） to mark proliferating cells of the hippocampal dentate gyrus Subgranular Zone（ SGZ）. Ghrelin was given intraperitoneal injection（ 80 μg/kg,once a day,continuous injection of 8 days）,the control group mice were offered same volume normal saline. After 1 week,both were immunohistochemical analysis. Results： Brd U-immunopositive cells widely expressed in the hippocampal dentate gyrus,the number of BrdU ＋ cells observed in Ghrelin treatment groups were significantly much more than that in control groups（ t =3. 43,P〈0. 01）. Conclution： Our results indicated that intraperitoneal injection of Ghrelin（ 80 μg/kg） induced neurogenesis in hippocampus.
出处
《泰山医学院学报》
CAS
2017年第10期1126-1128,共3页
Journal of Taishan Medical College
