摘要
建立益之康胶囊中总蒽醌含量测定的高效液相色谱法。采用HPLC测定益之康胶囊中2种蒽醌类成分大黄素和大黄素甲醚的含量;流动相为甲醇-0.1%磷酸溶液(75∶25,v/v),柱温30℃,流速1.0 m L/min,检测波长254 nm。大黄素和大黄素甲醚分别在0.054~0.65μg和0.022~0.27μg范围内呈良好的线性关系,相关系数分别为0.9999和0.9999,检出限分别为0.86μg/g和0.87μg/g,平均回收率分别为97.20%(RSD=2.26%)和99.96%(RSD=2.18%)。结论:该方法简便、准确、可靠。
Objective： To establish a High Performance Liquid Chromatography （ HPLC ） method for determining the totalanthraquinone in Yizhikang capsule.Methods ：The contents of emodin and physcion were determined by a HPLC method. The emodin and physcion were separated on a Waters Sunfire C l8 column （250 mm × 4.6 mm ,5 μm）using a mobile phase made up of methanol and 0.1% phosphoric acid solution（75：25 ,v/v） at a flow rate of 1.0 mL/min and detected by a UV detector at a wavelength of 254 nm.The column temperature was set at 30 ℃ .Results ：The response showed good linear relationship in the range of 54.09-649.05 Ixg/mL for emodin, 22.11 -265.31 μg/mL for physcion with all the coefficients of correlation （R2） = 0.9999. The detection limits（LODs） were 0.86 Ixg/g for emodin,0.87 μg/g for physcion, respectively.The average recoveries of emodin and physcion were 97.20% （ RSD = 2.26% ） and 99.96% （ RSD = 2.18% ）, respectively. Conclusions： This method is simple, accurate, reliable and reproducible and can be used for the quality control of yizhikang capsule.
作者
寿林均
程巧鸳
黄柳倩
周明昊
SHOU Lin-jun CHENG Qiao-yuan HUANG Liu-qian ZHOU Ming-hao(Zhejiang University of Technology, College of Chemical Engineering, Hangzhou 310014, China Zhejiang Institute for Food and Drug Control,Hangzhou 310052 ,China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第21期252-256,267,共6页
Science and Technology of Food Industry
