摘要
Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell（ESC）-mediated homologous recombination（HR）is time-and labor-consuming.
Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell（ESC）-mediated homologous recombination（HR）is time-and labor-consuming.
基金
supported by the Ministry of Science and Technology of China (2014CB964803 and 2015AA020307)
the National Natural Science Foundation of China (Nos. 31530048, 31601163 and 81672117)
he Chinese Academy of Sciences (XDB19010204 and QYZDJ-SSW-SMC023)
the Shanghai Municipal Commission for Science and Technology(16JC1420500, 17JC1400900 and 17140901500)
