摘要
目的:观察头针联合川芎嗪(TMP)对脑缺血再灌注模型大鼠神经功能缺损、脑梗死体积、皮层神经元损伤及MyD88表达的影响,探讨头针联合川芎嗪对脑缺血再灌注模型大鼠的脑保护作用及可能的作用机制。方法:165只大鼠随机分为假手术组(A组)、模型组(B组)、头针组(C组)、TMP组(D组)、头针+TMP组(E组)五组。每组根据再灌注时间分为IR1d(n=13)、IR3d(n=10)、IR7d(n=10)三亚组。采用改良的Zea Longa线栓法,制备右侧大脑中动脉缺血2 h再灌注(MCAO/R)大鼠模型;A组不中断大脑中动脉血流。C组和E组大鼠在右侧头部百会至曲鬓穴线进行透刺治疗;D组和E组分别给予腹腔注射TMP 20 mg/kg/次,每日一次。各组大鼠相应时间点进行神经功能评分测定;TTC染色检测再灌注1 d大鼠脑梗死体积;HE染色观察皮层神经元损伤及炎性细胞浸润情况;免疫组化、Western blot技术检测各组大鼠缺血侧脑组织MyD88表达水平。结果:E组大鼠在再灌注1 d、3 d、7 d,神经功能缺损程度均较其余四组明显减轻(P<0.05)。在再灌注1 d,E组大鼠相对脑梗死体积最小,与其余四组比较有统计学差异(P<0.05)。再灌注各个时间点,E组大鼠缺血侧皮层神经元损伤程度及炎性细胞浸润情况均较其余四组明显减轻;且缺血侧脑组织中MyD88阳性细胞数和相对蛋白含量均较其余四组明显降低(P<0.05)。结论:头针联合川芎嗪治疗可显著改善MCAO/R大鼠神经功能缺损,减少梗死体积,减轻皮层神经元损伤、炎性细胞浸润,具有脑保护作用,其机制可能与抑制缺血侧脑组织中MyD88蛋白表达、降低脑缺血再灌注后的炎症级联反应有关。
Objective： To observe the effects of scalp -acupuncture combined with Tetramethylpyrazine（TMP） on neuro- logical function, cerebral infarction size, cortical neuron injury and MyD88 expression in rats with cerebral ischemia reperfusion, and to explore the possible mechanism of its brain protection. Methods： 165 rats were randomly divided into sham operation group （ group A）, model group （ group B ）, scalp - acupuncture group （ group C ）, TMP group （ group D）, and scalp -acupuncture ＋ TMP group（ group E ）. According to different reperfusion time, each group was divided into three sub- groups, i.e. IRld（n = 13） , IR3d （n = 10）, and IR7d（n = 10）. The models were prepared by using the modified Zea Longa method of 2 h reperfusion（MCAO/R） in right medial cerebral artery. The blood flow of medial cerebral artery was not interrupted in group A; the rats in group C and E group were intervened with Baihui -Qubin scalp - acupuncture on the trouble side; and the rats in group D and group E were given 20 mg/kg TMP once daily. Scores of neural function at corresponding time points were evaluated; size of cerebral infarction was detected with TYC staining in 1 d rats; injury of cortical neurons and inflammatory cell infiltration were observed with HE staining; and ex- pression of MyD88 was detected by immunohistoy chemistry and Western blot method. Results： The degrees of neurological impairment in group E were significantly less than those of the other groups respectively after 1 d, 3 d and 7d reperfusion（P 〈0.05）. After 1 d reperfusion, the size of cerebral infarction was the smallest in group E, and there was a statistical difference compared to the other groups （ P 〈 0.05 ）. The injury degrees of ischemic and inflammatory cell infiltration group E were significantly lower than those in the other four groups, also the levels ~f MyD88 positive cells and the relative protein in the ischemic lateral brain tissue were significantly lower than those of the other four groups（P 〈 0. 05 ）. Conclusion： The therapy of scalp -acupuncture with TMP can significantly improve nerve function in the MCAO/R rats, reduce infarction size, decrease cortex neuron injury and inflammatory cells infiltration; it has the action of brain protection with the possible mechanism of inhibiting MyD88 expression and reducing inflammatory cascade reaction after brain ischemia reperfusion.
作者
吴明娟
孙晓伟
高延宏
卢金荣
赵岩
李锐
辛淑梅
程康陆
WU Ming - juan SUN Xiao - wei GAO Yan- hong LU Jin - rong ZHAO Yan LI Rui XIN Shu - mei CHENG Kang - lu(Heilongjiang TCM Academy of Sciences, Harbin 150036, China The First Affiliated Hospital of Heilongjiang University of Chinese Medicine ,Harbin 150040, China Harbin Yizhou Pharmaceutical Co Ltd,Harbin 150066, China Heilongjiang University of Chinese Medicine ,Harbin 150040, China)
出处
《中医药学报》
CAS
2017年第5期52-56,共5页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省自然科学基金(H2016045)
黑龙江省博士后资助项目(LHB-Z12248)
关键词
头针
川芎嗪
脑缺血再灌注损伤
髓样分化因子88
Scalp - acupuncture
Tetramethylpyrazine
Cerebral ischemia reperfusion injury
MyD88
