摘要
目的探讨20%高渗盐水对体外肿瘤细胞的灭活作用及温度、时间效应关系,为临床骨与软组织恶性肿瘤进行自体骨灭活再植保肢手术提供指导。方法选取四肢及骨盆术前活检确诊为骨与软组织恶性肿瘤患者24例,去除4例不符合条件病例,分三组分别在0.9%盐水、20%高渗盐水、巴氏法联合高渗盐水处理(15 min、30 min、45min),采用光镜、透射电镜观察并计算肿瘤细胞灭活率。结果 20%高渗盐水及巴氏法联合法各时间点肿瘤细胞灭活较0.9%等渗盐水存在明显差异,差异有统计学意义(P<0.01);巴氏联合法15min优于20%高渗盐水组(P<0.01)。结论 20%高渗盐水灭瘤作用确切,联合巴氏法或增加灭瘤时间能明显增加肿瘤细胞坏死率,增加灭瘤时间至30min以上灭瘤效果达到最佳。
Objective To study the effect of 20% hypertonic saline on the in vitro inactivation of tumor cells and its relationships with temperature and time,and to provide guidance for the inactivation of autologous bone and limb salvage operation in the treatment of clinical malignant tumors of bone and soft tissue.Methods 24 patients who were diagnosed with malignant tumors of bone and soft tissue according to the biopsy of limbs and pelvis before operation were chosen,and 4 patients who didn’t meet the requirement were removed.The samples were treated with 0.9% saline,20% hypertonic saline,and pasteurization treatment combined with hypertonic saline（15,30,45 min）and were observed by optical microscope,transmission electron microscope.The inactivation rate of the tumor cells were calculated.Results Inactivated tumor cells at 20% hypertonic saline and pasteurization combined with hypertonic saline at each time were obviously different compared with 0.9%isotonic saline,and the difference was statistically significant（P 〈0.01）.The effect of inactivating of 15 min pasteurization combined with hypertonic saline was better than 20% hypertonic saline group（P〈0.01）.Conclusion The effect of 20% hypertonic saline on tumor elimination is reliable,and combining with pasteurization or increasing the time of killing tumor cells can obviously increase the necrosis rate of tumor cells.The time of killing tumor cells is more than 30 min,and the effect of killing tumor will be best.
出处
《实用骨科杂志》
2017年第10期899-903,共5页
Journal of Practical Orthopaedics
基金
新疆维吾尔自治区自然科学基金资助项目(2014211C034)
关键词
高渗盐水
恶性骨肿瘤
灭活
凋亡
骨肉瘤
hypertonic saline
malignant bone tumor
inactivation
apoptosis
osteosarcoma
