摘要
对超高效液相色谱-串联质谱法测定猪肉中噁喹酸残留量的测量不确定度进行了分析。建立了测量过程中各个分量的数学模型,对测量过程中的不确定度来源进行逐项分析和合成,并估算了各不确定度分量对总不确定度的影响。结果表明:取包含因子k=2,置信概率为95%,待测猪肉样品中噁喹酸含量测量结果为(4.61±0.977)μg/kg时扩展不确定度U=k×U(X)=0.977μg/kg。影响噁喹酸含量测定不确定度有2个主要因素:一是来源于制作拟合噁喹酸标准曲线产生的不确定度,二是加标回收引起的不确定度。
An efficient ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method was established for the analysis of residues of oxolinic acid . The mathematical model of each component in the process of measurement was established,the sources of uncertainty were analyzed and synthesized item by item,and the effects of the uncertainty components on the total uncertainty were estimated. The results show-ed that the uncertainty of U= k xU （X ） was 0.977 pg/kg,when the content of oxolinic acid was （4.61±0.977） jxg./kg with a confidence probability of 95% and the coverage factor k = 2. There are tw-o main factors that affect the uncertainty of content deter^mination of oxolinic acid： the un-certainty derived from the calibration of the standard curye of the oxolinic acid and the uncertainty caused by the sample re-covery rate.
出处
《江苏农业学报》
CSCD
北大核心
2017年第5期1176-1181,共6页
Jiangsu Journal of Agricultural Sciences
基金
兵团农业科技攻关与成果转化项目(2015AC026)
