摘要
目的:建立杨梅中二种主要黄酮类成分的同时含量测定方法。方法:采用Cosmosil-5C18-PAQ色谱柱(250mm×4.6mm,5μm);流动相为乙腈–0.1%乙酸溶液梯度洗脱;流速1.0m L/min;二极管阵列检测器,检测波长520nm(1)和360nm(2),同时测定杨梅中矢车菊素-3-葡萄糖苷(1)、杨梅苷(2)的含量。结果:二种黄酮类成分色谱分离良好,线性范围依次为20.08~200.8g/m L(r=0.9996,1)、3.984~39.84 g/m L(r=0.9995,2),加样回收率依次为97.5%、96.2%。结论 :该测定方法快速准确,可为杨梅的质量分析方法提供参考。
Objective To establish an HPLC method for the simultaneous determination of two fiavonoids in the fruits of Myrico rubra. Methods The chromatographic separation was performed on a Cosmosil - 5C18 - PAQ column （250 mm 4. 6 mm, 5 μm） with a gradient elution of acetonitrile and 0. 1% acetic acid solution, at a flow rate of 1.0 mL/min, and detected at both 520 nm （for cyanidin -3 -gluco- side, 1 ） and 360 nm （for myricetrin 2） using diode array detector. Results The two compounds were separated with satisfaction, with lineari- ty ranges between 20. 08 -200. 8 g/mL （r =0. 9996, 1） and 3. 984 -39. 84 g/mL （r =0. 9995, 2）, and average recovery rates to be 97. 5% and 96. 2%. Conclusion The method is accurate and selective, which could be applied for the quality analysis of Myrico rubra.
作者
吴飞
李雅丽
顾田甜
马旗联
郝丽莉
刘江云
WU Fei LI Yali GU Tiantian MA Qilian HAO Lili LIU Jiangyun(College of Pharmaceutical Sciences, Suzhou University, Suzhou 215123, China The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China)
出处
《中国民族民间医药》
2017年第20期24-26,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
新疆维吾尔自治区自然科学基金(2015211C053)
