摘要
目的探讨孕妇脂肪量和肥胖相关基因(FTO)的表达与妊娠期胰岛素水平的相关性。方法分别选取2016年3～8月于珠海市妇幼保健院就诊的60例妊娠期糖尿病(GDM)孕妇和60例血糖正常孕妇作为研究对象,各空腹抽取2管静脉血。其中1管抗凝全血分离淋巴细胞提取RNA,并以RT-PCR法研究其淋巴细胞中FTO mRNA的表达水平;另1管非抗凝血离心取其血清,以酶联免疫法检测血清中FTO蛋白,化学发光法检测空腹胰岛素,自动生化分析仪检测血清GLU,CHO,TG,HDL-C和LDL-C等指标的浓度,并分析其相关性。结果病例组FTO mRNA,FTO蛋白水平、空腹血糖、OGTT 1 h和2 h血糖的结果分别为0.17%±0.10%,61.32±25.23 pg/ml,4.71±0.54 mmol/L,10.70±1.36 mmol/L和8.97±1.60 mmol/L,与对照组的0.11%±0.07%,51.47±22.97 pg/ml,4.41±0.28 mmol/L,8.05±1.04 mmol/L和6.56±0.75 mmol/L相比,差异均有统计学意义(t=3.876,2.236,3.817,11.964和10.578,P均<0.05)。病例组的胰岛素水平为6.83±9.76 mU/L,与对照组的13.15±13.99 mU/L相比,差异有统计学意义(t=-2.869,P<0.05)。另外,FTO mRNA与FTO蛋白,OGTT 1 h,2 h血糖水平呈正相关,与胰岛素水平呈负相关(均r=0.232,0.292,0.242,-0.185,P<0.05),与空腹血糖和血脂水平无相关性(P>0.05)。结论 GDM孕妇的FTO表达高于正常孕妇,FTO mRNA相对表达量与胰岛素呈负相关,其对血糖代谢的调节可能是通过影响胰岛素来实现。
Objective To evaluate the correlation of maternal FTO expression and insulin secretion levels in pregnant women. Methods In outpatient of Zhuhai Municipal Maternal and Child Healthcare Hospital,60 cases of pregnant women with ges tational diabetes mellitus （GDM） and 60 cases of healthy pregnant women were recruited in this study from March 2016 to August 2016. Each pregnant woman was taken two tubes of venous blood. Lymphocytes was isolated from one tube of anti- coagulated whole blood. The expression of FTO mRNA in lymphocytes was investigated using RT PCR. Serum was isolated from another tube of non-anticoagulant whole blood. Its FTO protein was investigated using ELISA,insulin was investigated using chemiluminescence method, and GLU,CHO, TG, HDL-C and LDL-C were investigated by automatic biochemical ana- lyzer. The correlation of the biochemical indicators were analyzed. Results The expression level of FTO mRNA, FTO pro rein, fasting glucose data, OGTT at lh and 2h glucose levels in the case group were 0.17 ± 0.10%, 61.32± 25.23 pg/ml, 4.71±0.54 mmol/L,10.70±1.36 mmol/L and 8.97± 1.60 mmol/L respectively. The values of these biochemical indica tots in control group were 0. 11±0.07%,51.47±22.97 pg/ml, 4. 41± 0. 28 mmol/L,8.05±1.04 mmol/L and 6.56＋± 0.75 mmol/L respectively. The differences were statistically significant（t= 3. 876,2. 236,3. 817,11. 964 and 10. 578,P± 0.05）. The insulin levels were 6.83±9.76 mU/L in the case group,and 13.15±13.99 mU/L in control group. The differences was statistically significant too（t=-2. 869 ,P±0.05）. FTO expression level was positively correlated with FTO pro tein,OGTT lh glucose and OGTT 2h glucose levels （r=0. 232,0. 292,0. 242,all P±0.05） ,and it was negatively correlated with insulin levels（r=-0. 185 ,P〈0.05）. There was no correlation between FTO and fasting glucose expression levels （P 〉0.05）. Conclusion The expression of FTO level in pregnant women with GDM was higher than that in healthy pregnant women. The relative expression of FTO mRNA was negatively correlated with insulin. And the regulation of glucose metab olism might be effected by insulin.
出处
《现代检验医学杂志》
CAS
2017年第5期44-47,共4页
Journal of Modern Laboratory Medicine
基金
珠海市医学科研基金项目(项目编号:2015J018)
关键词
妊娠期糖尿病
脂肪量和肥胖相关基因
胰岛素
gestational diabetes mellitus
fat mass and obesity associated gene
insulin
