摘要
目的探讨核转录因子(NF-κB)及肿瘤坏死因子(TNF-α)在儿童肺炎支原体肺炎(MPP)中表达水平及其辅助诊断价值。方法纳入高邮市中医医院、常州市儿童医院、宝应县妇幼保健院2016年01月～2017年01月收治的MPP患儿150例,根据症状将其分为喘息组(75例)及非喘息组(75例),同期匹配正常健康儿童(75例)作为对照组,收集不同组别儿童血液样本并提取血清后,通过实时荧光定量PCR检测比较三组人群血清中NF-κB及TNF-α的表达量差异。应用ROC曲线及AUC(95%CI)寻找其作为诊断指标的最佳临界值,同时评估其诊断效能。结果 MPP喘息组患儿血清中NF-κB及TNF-α的相对表达量分别为3.09±0.15和2.14±0.11,两者较正常对照组均升高,差异有统计学意义(t=11.03,6.987,均P<0.05)。MPP非喘息组患儿血清NF-κB及TNF-α的相对表达量分别为2.28±0.11和1.61±0.08,两者较正常对照组均升高,差异有统计学意义(t=6.873,7.406,均P<0.05)。喘息组患儿血清中NF-κB及TNF-α的相对表达量较非喘息组明显升高,差异有统计学意义(P均<0.05)。NF-κB诊断MPP的最佳临界值为1.895,其敏感度为71.33%,特异度为91.89%,AUC为0.8265,95%CI为0.772 8～0.880 2;TNF-α诊断MPP的最佳临界值为1.450,其敏感度为63.00%,特异度为90.54%,AUC为0.818 3,95%CI为0.765 1～0.871 6。结论 MPP患儿血清中NF-κB,TNF-α的表达量均较正常对照组升高,血清NF-κB及TNF-α可作为儿童MPP的辅助诊断指标。
Objective To explore the diagnostic value of the quantitative detection of serum NF-κB and TNF-~ in children with Mycoplasrna pneumoniae Pneumonia（MPP）. Methods 150 MPP patients were enrolled in this study including 75 with wheeze and 75 without wheeze, and then collected the blood specimens. 75 healthy children were recruited to the control group （NC group）. The levels of NF-κB and TNF-α in different groups were detected by qRT-PCR. The performance of NF- κB and TNF-α were evaluated by receiver operating characteristic curves （ROC） and the area under the curve （AUC） （95 CI）. Results The relative expression of NF-gB and TNF-α in MPP children with wheeze were 3.09 ± 0. 15 and 2.14± 0.11, respectively. They were significantly higher than NC group （t= 11.03,6. 987, P^0.05）. NF-κB and TNF-α in MPP children without wheeze were also significantly higher than control group （t= 6. 873,7. 406, P^0.05）. The relative expression of NF-κB and TNF-α in children with wheeze were significantly higher than children without wheeze. The detection of NF-κB yielded an area under the curve of ROC of 0. 826 5 （95%CI：0. 772 8-0. 880 2） in discriminating MPP patients from healthy group. The TNF-α was 0. 818 3 （95 % CI：0. 765 1-0. 871 6） in discriminating MPP patients from healthy group. Conclusion The relative expression of NF-gB and TNF-α in children with MPP was significantly higher than that in NC group,NF-κB and TNF-α may prove to be non-invasive biomarkers for the auxiliary diagnosis of MPP.
出处
《现代检验医学杂志》
CAS
2017年第5期87-89,93,共4页
Journal of Modern Laboratory Medicine
