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ILT4促进脓毒症炎性反应并介导免疫麻痹 被引量:1

Immunoglobulin-like transcript-4 (ILT4) expression in mononuclear cells on the prognosis of sepsis
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摘要 目的:探讨脓毒症单核细胞表达ILT4的生物学行为和效应,及对于预后的影响。方法:选取BALB/c、BALB/c ILT4–/–雄性小鼠,CLP复制脓毒症模型。用流式细胞仪定量检测CLP术后24h单核细胞ILT4、MHC-Ⅱ表达水平;用ELISA法检测各组0、6、12、24h血清IL-6、TNF-α浓度;并观察168h内生存预后。 结果:CLP术后24小时脓毒症小鼠外周单核细胞高度表达ILT4分子 (193.50 ± 52.54 vs. 1292.00 ± 143.70, p 〈 0.05) ;较WT组ILT4–/–小鼠外周单核细胞表达MHC-Ⅱ比率明显增高(24.25± 6.76% vs. 49.38 ±5.66%, p 〈 0.05) 。CLP术后24h血清IL-6显著增高(54.25± 20.04 vs. 470.75±88.03, p 〈 0.05),ILT4敲除后很大程度的抑制这一趋势(470.75±88.03 vs. 241.25 ± 45.10, p 〈 0.05);但对TNF-α表达无显著性干扰(53.13 ±5.49 vs. 50.88 ±6.38, p〉 0.05)。且ILT4–/–小鼠CLP术后生存率WT明显增加(p 〈 0.05)。结论:脓毒症时单核细胞高表达ILT4,与高IL-6水平及低MHC-Ⅱ表达率相关,导致死亡率增加。 Objective To study the biological behaviors and effects of immunoglobulin-like transcript-4 (ILT4) expression in monormclear cells on the prognosis of sepsis. Methods ILT4 +/+ (WT) and ILT4-knockout mice (ILT4-/-) male BALB/c mice were used for sepsis modeling using cecal ligation puncture (CLP). Flow cytometry was used to measure the levels of expression of ILT4 and major histocompatibility complex class Ⅱ molecules ( MHC-Ⅱ ) in mononuclear cells of peripheral blood 24 h after CLP. ELISA was used to measure the concentrations of intedeukin-6 (IL-6) and serum tumor necrosis factor-alpha (TNF-α) in different groups of mice at 0 h, 6 h, 12 h, and 24 h after CLP to monitor the survival and prognosis over the course of 168 h. Results ILT4 was highly expressed in mononuclear cells of the peripheral blood of septic mice 24 h after CLP in comparison with that before CLP (1292.00±143.70) vs. ( 193.50±52. 54) , P 〈 0.05. MHC- Ⅱ expression in mononuelear cells of the peripheral blood in ILT4-/- mice was significantly higher than that in WT mice (49.38±5.66)% vs. (24.25±6.76) % , P 〈 0. 05 ). Serum IL-6 was significantly elevated 24 h after CLP compared with that before CLP (470. 75±88.03) vs. (54. 25±20.04), P 〈 0. 05. The serum IL-6 concentration was much lower in ILT4-/- mice than that in MT mice (241.25±45.10) vs. (470.75±88.03), P 〈 0.05; whereas, there was no significant difference in TNF-ct expression between two groups of mice (50. 88±6. 38 ) vs. (53.13±5.49), P 〉 0. 05. The survival rate of ILT4-/- mice was significantly higher after CLP compared with WT mice (P 〈 0. 05 ). Conclusion The high level of ILT4 expression in mononuclear cells were observed in peripheral blood during sepsis and it was found to be associated with high serum IL-6 levels and low MHC-Ⅱ expression in mononuclear cells, leading to increased mortality.
出处 《中华急诊医学杂志》 CAS CSCD 北大核心 2017年第10期1160-1163,共4页 Chinese Journal of Emergency Medicine
基金 国家自然科学基金(81272138) 上海市卫计委课题(20124349)
关键词 脓毒症 单核细胞ILT4 CD85 MHC-Ⅱ IL-6 TNF—α 免疫麻痹 盲肠结扎穿孔术 Sepsis Monoyte Immunoglobulin-like transcript-4 ( ILT4 ) CD85 Major histocompatibility complex class Ⅱ molecules ( MHC- Ⅱ ) Intedeukin (IL) -6 Tumor necrosis factor- alpha (TNF-α) Immunologic paralysis Cecal ligation puncture
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