摘要
目的研究PJA1及miR-130a在口腔肿瘤组织中的表达情况,明确对肿瘤细胞迁移增殖的影响,并探究两种基因之间的相互作用。方法收集15组口腔肿瘤及其瘤旁组织,提取组织内RNA,应用qRT-PCR法检测组织中PJA1及miR-130a的表达情况;构建PJA1过表达、miR-130a过表达及两者同时过表达的口腔肿瘤细胞株SCC-3,应用MTT实验和Wound scratch assay实验分别检测细胞增殖与迁移的影响。结果 qRT-PCR结果表明PJA1在口腔肿瘤组织中的表达明显低于瘤旁组织(P<0.05),miR-130a在口腔肿瘤组织中的表达明显高于瘤旁组织(P<0.05);MTT和Wound scratch assay实验结果表明PJA1过表达可以抑制肿瘤细胞的增殖与迁移,miR-130a过表达可以促进肿瘤细胞的增殖与迁移,两者同时过表达PJA1可以抑制miR-130a促进肿瘤细胞增殖与迁移的能力。结论 PJA1在口腔肿瘤组织中低表达,具有抑制肿瘤细胞增殖和迁移的能力;miR-130a在口腔肿瘤组织中高表达,具有促进肿瘤细胞增殖和迁移的能力;miR-130a可能通过抑制了PJA1从而促进肿瘤细胞的增殖和迁移。
Objective To study the expression level of PJA1 and miR-130a in oral tumor tissue and to determine the effect on tumor cell migration and proliferation, and to explore the interaction between the two genes. Methods 15 groups of oral tumor and adjacent tissues were collected and their mRNA was extracted. The expression level of PJA1 and miR-130a was detected by qRT-PCR. Overexpression of HA1, overexpression of miR-130a, overex- pression of both PJA1 and miR-130a in SCC-3 were constructed. The effect of cell proliferation was examined by MTr assay, and the effect of cell migration was determined through Wound scratch assay. Results qRT-PCR re- vealed that the expression of HA1 in oral tumor tissues was obviously lower than that in adjacent tissues （P 〈 0.05 ）, the expression of miR-130a in oral tumor tissues was significantly higher than that in adjacent tissues（P 〈 0.05 ）. MTY and Wound scratch assay demonstrated that overexpression of PJA1 could inhibit the proliferation and migration of SCC-3, overexpression of miR-130a could promote the proliferation and migration of SCC-3. PJA1 could inhibit the ability of miR-130a to promote the proliferation and migration of SCC-3 when both of them were overexpressed at the same time. Conclusion The expression level of PJA1 is down-regulated in oral tumor tissues, PJA1 can inhibit tumor cell proliferation and migration. The expression level of miR-130a is up-regulated in oral tumor tissues, miR-130a can promote tumor cell proliferation and migration. MiR-130a may promote oral tumor cell proliferation and migration by inhibiting PJA1.
出处
《安徽医科大学学报》
CAS
北大核心
2017年第11期1622-1626,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:31501103)
安徽省自然科学基金(编号:1508085QC62)
