摘要
目的探讨法舒地尔（Fasudil）在小鼠脑缺血再灌注（Ischemia／reperfusion，I／R）损伤中的神经保护作用。方法51只c57BL／6J小鼠随机分为两组，羟甲基纤维素（carboxymethylcellulose，CMC）处理组（26只）和Fasudil处理组（25只）。首先给予Fasudil（10mg／kg）或CMC（0．5％CMC10ml／kg）处理，然后行脑I／R动物模型缺血60min后再灌注18h。氯化三苯四氮唑染色法（2，3，5-triphenyhetrazoliu mchloride，rrc）染色分析脑梗死情况，伊万斯蓝（Evansblue）和白蛋白（Albumin）免疫印迹分析血脑屏障通透性的改变，酶谱法检测MMP9活性变化。结果在小鼠脑I／R动物模型中CMC组的脑坏死体积为（99．07±6．53）mm3，Fasudil组的脑坏死体积为（57．02±8．93）mm。（P〈0．01）；Fasudil处理组I／R小鼠血液中MMP9活性低于CMC处理组；与CMC处理组相比，Fasudil处理组的脑组织中Albumin和Evansblue相对含量分别从（2．95±0．77），（5．15±0．24）减少到（1．04±0．18），（1．96±0．31）（均P〈0．01）。结论Fasudil通过抑制MMP9活性以维持血脑屏障通透性，从而保护I／R损伤。
Objective To investigate the neuroprotective effects of Fasudil in cerebral I/R injury in mice. Methods 51 C57BL/6J mice was divided into two grnups,CMC treated group （n=26） and Fasudil treated group （n=25） ,randomly. The mice were treated with Fasudil （10 mg/kg） or CMC （0.5% CMC 10 ml/kg） separately. Then the treated mice were subjected to 60 min of focal ischemia and 18 h reperfusion. The infarct volume of brain was analyzed by TTC staining with MCID image system. BBB permeability was assessed by Evans blue extravasation and albumin leakage which was detected by immuno-blotting assay. The activity of MMP9 was analyzed by zymography. Results The infarct volume in CMC group （（99.07±6.53） mm3） was larger than that in Fasudil group （（57.02±8.93）mm3） ,the difference was statistically significant （P〈0.01） .The activity of MMP9 in the mice treated with Fasudil was lower than that in CMC group. Com- pared with the CMC group（ albumin （2.95±0.77）,Evans blue （5.15±0.24））, the albumin and Evans blue content in the Fasudil treated group （ albumin （ 1.04 ± 0.18 ）, Evans blue （ 1.96 ± 0.31 ） ） reduced significanctly （all P〈 0.01 ）. Conclusion Fasudil protects I/R damage by inhibiting the activity of MMP9 to maintain blood-brain barrier permeability.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2017年第10期933-937,共5页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目（81360206）
云南省中青年学术技术带头人后备人才基金项目（2014HB025）
云南省教育厅科研基金项目（2015Y379）
