摘要
采用水提醇沉法从杜香(Ledum palustre L.)叶中提取得到多糖,通过硫酸苯酚比色法测定杜香叶多糖含量;在单因素试验的基础上进行正交试验优化提取条件,采用试剂盒法和化学实验法测定其体外总抗氧化活性以及DPPH·、·OH和H2O2清除能力;采用肝癌细胞Hep G2为细胞模型,研究其对Hep G2细胞的体外抗增殖活性。结果表明:杜香叶多糖提取的最佳条件为:液料比30∶1(m L∶g),提取温度80℃,提取时间3 h。在此条件下,多糖提取得率为7.86%±0.16%,纯度为90.06%。在质量浓度1~5 g/L范围内,杜香叶多糖体外抗氧化活性和抗癌细胞增殖活性均呈现出量效关系趋势。5 g/L杜香叶多糖总抗氧化能力为(10.27±0.2)U/m L,达到相同质量浓度的Vc总抗氧化活性的95%以上;各浓度杜香叶多糖DPPH·、·OH清除能力均达到相同质量浓度的Vc 90%以上,杜香叶多糖对H2O2清除能力在5 g/L时达到相同质量浓度的Vc 60%以上;当质量浓度为5 g/L时,杜香叶多糖对Hep G2细胞增殖抑制率达57.41%±0.02%。
The polysaccharide from Ledum palustre L. leaves was obtained by ethanol precipitation,and its content was determined by phenol-sulfuric acid assay. Based on the single-factor test,a orthogonal test was designed to optimize the extracting conditions.The total antioxidant activity and the scavenging effect on DPPH free radical,OH free radical and H2 O2 by the polysaccharide from L. palustre leaves in vitro were determined by the kit method and chemical method. The antiproliferative activity was evaluated on human liver cancer Hep G2 cells in vivo. The results showed that the best extracting conditions of the polysaccharide were： the liquid-solid ratio 30∶ 1（ v/w）,extraction temperature 80 ℃ and extraction time 3 h. Under these conditions,the extraction yield of the polysaccharide was 7. 86% ± 0. 16% and the purity reached 90. 06%. In the range of 1-5 g/L,the antioxidant and antiproliferative activities increased with an increase of mass concentration. At the mass concentration of 5 g/L,the total antioxidant activity was（ 10. 27 ± 0. 2） U/m L and reached more than that of 95% Vc with the same mass concentration. The scavenging effects on DPPH free radical and OH free radical in different concentration reached more than those of 90% of Vc with the same mass concentration. The scavenging effect on H2 O2 reached more than that of 60% Vc with the same mass concentration and the proliferation inhibitory rate of Hep G2 cells was 57. 41% ± 0. 02% at the mass concentration of 5 g/L.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2017年第5期133-138,共6页
Chemistry and Industry of Forest Products
基金
国家级大学生创新训练项目资助(201510225154)
关键词
杜香
多糖
抗氧化
抗肿瘤
Ledum palustre L.
polysaccharide
antioxidant
anti tumor
