摘要
为调查高寒牧区引起奶牛乳房炎的凝固酶阴性致病葡萄球菌流行现状及耐药情况,本研究对呼伦贝尔地区3个规模化奶牛养殖场采用加州乳房炎测试法(CMT)进行高体细胞牛脂病检测,并采集330份牛奶样品,应用PCR方法、脉冲场凝胶电泳(PFGE)分型和药敏试验等对样品中的葡萄球菌进行分离鉴定和耐药性分析。结果显示,该地区隐性乳房炎发病率达47.8%,共分离和鉴定出9种葡萄球菌60株(34.5%);60株葡萄球菌分为41个克隆谱型;60株葡萄球菌对青霉素和林可霉素的耐药率较高,分别为40.0%(24/60)和50.0%(30/60),表明这两种药是治疗乳房炎常用药物。本研究为高寒地区致病葡萄球菌引起的奶牛乳房炎临床合理用药及对病原菌的防控提供实验依据和指导。
To investigate the prevalence and drug resistance of coagulase-negative Staphylococcus from mastitis of dairy cow in Hulunbuir, a total of 330 milk samples were collected from dairy cows with a high somatic cell disease tallow （using the California mastitis test method, CMT） from three large-scale farms, during the period May to September 2015. Staphylococcus spp strains were isolated and identified by PCR, pulsed field gel electrophoresis （PFGE） typing and antimicrobial susceptibility testing, respectively. The results showed that 60 Staphylococcus spp strains belonging to 9 different genera and 41 clone-types were obtained, and the isolation rate was 47.8%. The 13 different genera Staphylococcus included 4 main pathogenic staphylococci （114 strains, 65.5%） and 9 opportunistic Staphylococci （60 strains, 34.5%）. These Staphylococcus isolates showed resistant rate to penicillin （40.0%, 24/60） and clindamycin （50.0%, 30/60）, suggesting that the two antibiotics had been commonly used in this region. In this study, rational antibiotic use and pathogens prevention and control strategies were developed via above-mentioned studies.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2017年第10期848-851,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
十三五国家重点研发计划"畜禽病原菌耐药性数据库建设及预警技术研究"(2016YFD0501304)
关键词
葡萄球菌
乳房炎
药敏试验
PFGE分型
Staphylococcus aureus
mastitis
antimicrobial susceptibility test
PFGE typing
