摘要
目的应用基因测序技术检测武汉地区慢性乙型肝炎患者的乙型肝炎病毒(HBV)基因型并分析其耐药情况。方法收集2015年1月—2016年12月武汉大学人民医院感染科263例乙型肝炎病毒基因(HBV-DNA)阳性的慢性乙型肝炎患者血浆标本,通过Sanger测序法测定HBV P基因RT区的基因序列,与NCBI genebank数据库中HBV基因型数据进行比对,分析患者HBV基因型。同时分析P基因RT区的突变位点和耐药情况。结果共检测出5种HBV基因型,分别是B型181例(68.82%),C型76例(28.90%),D型3例(1.14%),B/C混合型1例(0.38%)和B/F/G混合型2例(0.76%)。共检出耐药株109例(41.44%),HBV B基因型耐药突变发生率高于C基因型,以L180M+M204V/I和M204V/I耐药模式最为常见。结论武汉地区HBV基因型以B型为主,其次为C型;耐药形式复杂多样,耐药发生率与基因型别存在一定关系。
[Objective]To detect the genotype and drug resistance of hepatitis B virus in chronic hepatitis B patients in Wuhan by gene-sequencing technology.[Methods]A total of 263 HBV-DNA-positive plasma samples were collected from chronic hepatitis B patients in infectious diseases department of Renmin hospital of Wuhan university from Jan. 2015 to Dec. 2016,then gene sequence of HBV P gene RT area were detected by Sanger sequencing method and compared with HBV genotype in NCBI genebank database for analyzing HBV genotype. At the same time,the mutant sites and drug resistance of P gene RT area were analyzed.[Results]A total of 5 kinds of HBV genotypes were detected,including 181 cases（68.82%）of genotype B,76 cases（28.90%）of genotype C,3 cases（1.14%）of genotype D,1 cases（0.38%）of genotype B/C,and 2 cases（0.76%）of genotype B/F/G. A total of 109 cases（41.44%）of drug resistant strains were detected. The incidence of drug resistance of HBV B genotype was higher than that of C genotype. The drug resistance forms of L180M＋M204V/I and M204V/I were the most common.[Conclusion]Genotype B is the major type of HBV-DNA genotype,followed by genotype C. The forms of drug resistance are complex and diverse,and the incidence of drug resistance and genotype has a certain relationship.
作者
吴泽刚
李艳
童永清
WU Ze-gang LI Yon TONG Yong-qing(Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Hubei Wuhan,430060,Chin)
出处
《职业与健康》
CAS
2017年第19期2718-2720,共3页
Occupation and Health
基金
国家临床重点专科建设项目(财社[2010]305号)
关键词
乙型肝炎病毒
基因测序
基因分型
耐药
Hepatitis B virus
Gene sequence
Genotype
Drug resistance
