摘要
目的 :研究黄芪液对乳酸盐腹透液致腹膜间皮细胞增殖及损伤作用的影响。方法 :采用胰蛋白酶 -ED TA消化法 ,从人腹膜组织中分离间皮细胞 ,并建立体外人腹膜间皮细胞 (HPMC)培养模型。以MTT法测定HPMC增殖程度 ,采用自动生化分析仪检测 ,培养液中乳酸脱氢酶 (LDH)水平示细胞损伤程度。结果 :2 .5 %乳酸盐腹透液中加用黄芪液 (1mg/ml)与HPMC作用 4 5min ,其培养液中LDH水平较腹透液对照组明显降低(P <0 .0 1) ,HPMC增殖能力明显增强 (P <0 .0 1)。结论 :黄芪液能拮抗乳酸盐腹透液致HPMC的损伤。
Objective:To determine the effects of huangqi injection on the cytotoxicity of lactate-buffered peritoneal dialysis solutes(L-PDS) to human peritoneal mesothelial cells (HPMC).Methods:HPMC was separated with trypsin-EDTA.HPMC proliferation and the concentration of lactate dehydrogenase (LDH) in supernatants were detected by MTT assay and automatic biochemical analyzor,respectively.Results:The addition of 1mg/ml of huangqi to 2.5% L-PDS caused an increase in HPMC proliferation, and a decrease in release of LDH for exposure 45min( P <0.01).Conclusion:Huangqi injection can reverse the injury of L-PDS to HPMC.
出处
《中国现代医学杂志》
CAS
CSCD
2002年第16期32-33,39,共3页
China Journal of Modern Medicine
基金
湖南省自然科学基金项目 (0 1JJY2 0 71)
湖南省卫生厅基金项目 (2 0 0 1-Y -3 9)
