摘要
[目的]评价ITS2与psbA-trnH条形码候选序列对含有小檗碱的药用植物的鉴定作用。[方法]对含有小檗碱与不含有小檗碱的药用植物的ITS2与psbA-trnH序列进行PCR扩增和测序,进行种内和种间变异分析,构建NJ进化树评价不同序列的鉴定能力。[结果]ITS2与psbA-trnH序列均能够成功地区分含有小檗碱与不含有小檗碱的药用植物。然而,ITS2在区分含有小檗碱的不同科药用植物的效率优于psbA-trnH序列。[结论]建立了以ITS2序列为主,psbA-trnH序列为辅的鉴定含有小檗碱的药用植物的方法,这为含有小檗碱的药用植物种质资源的整理与标准化,甚至育种都提供了有效的基础数据。
[Objective]To evaluate and construct alternative molecular method to identify the medicinal plants containing berberine. [Methods] Total genomicDNA was extracted from medical plants that contain berberine and not contain berberine,respectively. The ITS2 and psbA-trnH sequences were obtained after PCR amplification and bidirectional sequencing. The variable sites and neighbor-joining（ NJ） phylogenetic trees were computed and constructed by MEGA 6. 0. [Results]The medical plants that contain berberine and not contain berberine might be clustered into two branches based on ITS2 and psbA-trnH sequences. However,ITS2 was better than psbA-trnH for the identification of medical plants containing berberine from various families. [Conclusion] The identification method of medical plants containing berberine was established based on ITS2 and psbA-trnH sequences,which provided basis for the arrangement,standardization and even breeding of the medicinal plants containing berberine.
出处
《生物技术》
北大核心
2017年第5期452-459,439,共9页
Biotechnology
基金
国家自然科学基金项目("决明胰蛋白酶抑制剂调控菜青虫中肠消化酶合成的分子机制研究"
No.31500276
"藏医抗脑缺血名方药效物质的神经保护机制研究"
No.81473337)
国家重大专项("重大新药创制-中药新药安全性检测技术与标准研究"
No.2014ZX09304-307-001-019
"重大新药创制-葛黄有效组分防治2型糖尿病候选药物研究(注册分类第5类)"
No.2013ZX09103-002-014)
四川省应用基础研究项目("决明橙黄决明素生物合成关键酶基因的筛选与鉴定"
No.2017JY0222)
成都市科技技术研发项目("基因工程酶转化合成阿魏酸的关键技术研究"
No.2015-HM01-00051-SF)
国家级大学生创新创业训练计划资助项目("基于DNA条形码的芸香科植物黄柏鉴定"
No.201610613070)
