摘要
为了考察中草药添加剂对公猪精液品质、血清激素及基因表达的影响,试验将24头日龄、体况相近的大约克种公猪随机分为3组,对照组饲喂基础日粮,试验1组饲喂基础日粮+2%中草药添加剂,试验2组饲喂基础日粮+3%中草药添加剂,测定公猪精液量、精子畸形率、精液密度、精子活力、促卵泡生成素(FSH)、促黄体生成素(LH)和睾酮(T)含量以及公猪雄性相关基因(GAPDH、CREB、CYP19)表达量等相关数据。结果表明:与对照组相比,试验1,2组精液量有所提高;试验1,2组精子畸形率均低于对照组,其中试验2组显著降低(P<0.05);试验1,2组精子密度、精子活力高于对照组,其中试验2组显著高于对照组(P<0.05)。与对照组相比,试验1,2组FSH及试验2组LH、T含量显著增加(P<0.05);试验2组CREB基因表达量比对照组上调了2.66倍(P<0.05),CYP19基因表达量上调了4.63倍(P<0.01)。说明饲粮中添加中草药添加剂可以通过调节激素水平改善精液品质,从而提高种公猪精子活力、密度,降低畸形率;3%中草药添加剂效果更佳。
In order to investigate the effect of Chinese herbal medicine supplement on boar semen quality, serum hormone and gene expression, twenty - four Durocboars with similar age and body conditionswere randomly divided into three groups. Control group was fed with basic diet, NO. 1 group with basic diet supplemented with 2% Chinese herbal medicine, and NO. 2 group with basic dietsupplemented with 3% Chinese herbal medicine. Several factors were determined including semen volume, sperm deformity rate, semen density, sperm viability, the contents of follicle - stimulating hormone （ FSH ） , luteinizing hormone （ LH ） andtestosterone （T） , andexpression levels of boar male - related genes （ GAPDH, CREB ,CYP19 ）. The results showed that experimental groups NO. 1 and 2 had an increased semen volumes and lower sperm deformity rates compared with control, with group NO. 2 having a significantly lower rate（ P 〈 0.05 ）. Semen densityand sperm viability were higher in groups NO. 1 and 2 than those in control,with group NO. 2 having a significantly higher rate（P 〈 0.05 ）. Compared to control group, FSH content in groups NO. 1 and 2or LH content and T content in group NO. 2, increased significantly （ P 〈 0.05 ）. Compared with control group, CREB and CYPI9 expression levels up - regulated 2.66 folds（ P 〈 0.05 ） and 4.63 folds（ P 〈 0.01 ） in group NO. 2, respectively. The results suggested that Chinese herbal medicine supplemented in diet could improve semen quality by regulating hormone contents ,and could enhance boarsperm viability,semen density,and meanwhile could reduce sperm deformity rate. Diet supplemented with 3% Chinese herbal medicine had better effect on boar.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2017年第11期138-140,148,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31272606)
关键词
公猪
中草药添加剂
精液品质
血清激素
基因表达
boar
Chinese herbal medicine supplement
semen quality
serum hormone
gene expression
