摘要
目的明确幽门螺杆菌(heliobacter pylori,Hp)对克拉霉素的耐药情况进而探讨Hp对克拉霉素耐药机制。方法分离培养获得96株Hp。采用琼脂稀释法进行体外抗生素敏感试验,检测Hp对克拉霉素的最低抑菌浓度(minimalinhibitory concentration,MIC),拟定克拉霉素MIC>2μg/m L为耐药菌株。比较城市和农村的差异。挑选克拉霉素耐药Hp10株,提取细菌DNA,用PCR-限制性片段长度多态性(RFLP)分析探讨克拉霉素耐药机制。结果 Hp对克拉霉素的耐药率为25%,城市和农村耐药率差异无统计学意义(P>0.05)。16株克拉霉素耐药菌株23S r RNA基因功能区V PCR扩增片段,均被BsaⅠ酶切,未被BbsⅠ酶切,提示16株在2143位点有A→G突变。结论 Hp对克拉霉素的耐药率较高,且城市和农村无显著差异。大多数克拉霉素耐药Hp存在23Sr RNA基因功能区V 2143位点A→G突变。
Objective Toclarify the resistance of Helicobacter pylori（Hp） to clarithromycin and to explore the mechanism.Methods 96 strains of Hp were obtained by isolation and culture.The minimal inhibitory concentration（MIC） of Hp to clarithromycin was determined by agar dilution method.Clarithromycin resistant strains were determined by MIC 2μg/m L. The difference between urban and rural was compared.The mechanism of clarithromycin resistance was analyzed by PCR-restriction fragment length polymorphism（RFLP） through selecting 10 Clarithromycin resistant Hp strains and extracting their gene. Results The resistance rate of Hp to clarithromycin was 25%,and there was no significant difference in resistance rate between urban and rural areas（P〉0. 05）.In the 16 Clarithromyc resistant strains,the PCR amplification fragment of gene functional domain Vof 23 S r RNA were digested by BsaⅠ,but not BbsⅠ,suggesting that there were A → G mutation on the 2143 site in the 16 strains.Conclusion Hp has a higher resistance to clarithromycin,and there is no significant difference between urban and rural areas.Most clarithromycin-resistant Hps present A → G mutation on gene functional domain V2143 site of 23 S r RNA.
出处
《四川医学》
CAS
2017年第10期1143-1146,共4页
Sichuan Medical Journal
关键词
幽门螺杆菌
克拉霉素
耐药性
突变
helicobacter pylori
clarithromycin
resistance
mutation
