摘要
目的观察苦参素联合顺铂对人肝癌裸鼠皮下移植肝癌miR-21、miR-122表达的影响,探讨其可能的分子机制。方法成功构建BALB/c裸鼠人肝癌细胞株HepG2细胞移植瘤模型后,随机分为对照组、苦参素组、顺铂组及苦参素和顺铂联合用药组(每组12只裸鼠,共48只)。分别给药,对照组(生理盐水10mg/kg)、苦参素组(苦参素100mg/kg)、顺铂组(顺铂2mg/kg)、联合组(苦参素100mg/kg+顺铂2mg/kg)。每天1次,均为腹腔注射给药。分别记录对照组、顺铂组、苦参素组和联合用药组在4、7、9、11以及14天裸鼠的体重和肿瘤体积,并计算抑瘤率。同时,应用实时荧光定量RT-PCR法检测皮下移植瘤miR-21和miR-122的表达。结果第14天联合用药组裸鼠体重明显大于顺铂组(P<0.01),第14天裸鼠肿瘤体积在联合用药组明显小于顺铂组(P<0.01)。与正常对照组比较,三组miR-21表达下调,miR-122表达下调。三组miR-21的表达分别为53.45±12.89、6.92±3.12和0.66±0.02,差异具有统计学意义(P<0.01)。三组miR-122表达分别为43.6±12.41,20.4±6.31以及0.22±0.09,差异具有统计学意义(P<0.01)。结论苦参素和顺铂联合用药对裸鼠皮下肿瘤有明显抑癌作用,其机制可能是调节miR-21和miR-122的表达抑制肝癌细胞过度增殖,从而发挥抗肿瘤作用。
Objective To investigate whether combined oxymatrine and cisplatin have a influence on expression of miR-21,miR-122 in experimental hepatic carcinoma and to discuss potential molecular mechanism. Methods BALB/c nude mice constructed with hepatocellular carcinoma were randomly divided into control group,oxymatrine group,cisplatin group and combination group. Control group（ 10 mg/kg）,matrine group（ 100 mg/kg）,cisplatin group（ 2 mg/kg）,combined group（ matrine 100 mg/kg ＋ cisplatin 2 mg/kg） were treated with 1 time/day by intraperitoneal injection,respectively. The tumor size,weight and tumorous inhibitory rate of four groups were recorded on 4,7,9,11 and14 days. Real-time fluorescent quantitative RT-PCR method was used to measure the expression of miR-21,miR-122 in experimental hepatic carcinoma. Results Nude mice weight in combination groups was greater than cisplatin group and the tumor volume of nude mice was smaller than that of cisplatin group（ P〈0. 01） on 14 days（ P〈0. 01）. The expression of miR-21 and the expression of miR-122 was down-regulated compared with control group. The expression of miR-21 was 53. 45 ± 12. 89,6. 92 ± 3. 12 and 0. 66 ± 0. 02,and the expression of miR-122 was 43. 6 ± 12. 41,20. 4 ± 6. 31 and 0. 22 ± 0. 09,respectively. The differences had statistically significance（ P〈0. 01）. Conclusion A remarkable tumorous inhibitory rate was observed in combined oxymatrine and cisplatin groups. Anti-tumor role may adjust the expression of miR-21 and miR-122 to inhibit excessive proliferation of tumour cell.
出处
《临床和实验医学杂志》
2017年第21期2084-2087,共4页
Journal of Clinical and Experimental Medicine
基金
广西自然科学基金资助项目(2014GXNSFAA118143
0542119)
广西高校优秀人才基金资助项目(20077029)
广西卫生厅课题基金重点基金资助项目(200887)
