摘要
目的探讨雷利度胺对Molm-13急性单核细胞白血病细胞体外增殖与凋亡的影响。方法以噻唑蓝法检测不同浓度雷利度胺(60、120、180、240、300 mg/L)在不同的时段(24、48、72 h)处理Molm-13细胞后其增殖率的变化;不同浓度的雷利度胺(120、180、240 mg/L)作用Molm-13细胞48 h后,流式细胞仪检测细胞凋亡率的变化,荧光显微镜观测细胞凋亡形态学的变化。结果雷利度胺处理Molm-13细胞48 h后,Molm-13细胞出现浓度依赖性凋亡率和凋亡形态学变化。结论雷利度胺可抑制Molm-13细胞增殖,促进细胞凋亡,其作用具有浓度依赖性及时间依赖性。
Objective To observe the anti-acute monocytic leukemia effect of lenalidomide in vitro.Methods The inhibitory effect of lenalidomide on Molm-13 cells was estimated with MTT assay,and different action times（24,48,72 h） and lenalidomide concertrations（60,120,180,240,300 mg/L） were adopted.The morphological changes of the cells were observed under a fluorescence microscope.Cell apoptosis was analyzed by flow cytometry.Results Proliferation of Molm-13 cells were inhibited after being treated with lenalidomide,in a time-and dose-dependent manner.Treated with lenalidomide,cells morphological changes of apoptotic could be observed under a fluorescence microscope and the apoptosis rate also was elevated.Conclusion Lenalidomide could moderately inhibit Molm-13 cell proliferation and induce cell apoptosis in a time-and dose-dependent manner.
作者
郭长安
李明玉
郝相勇
李永红
李红玲
Guo Chang-an Li Ming-yu Hao Xiang-yong Li Yong-hong Li Hong-ling(Department of Oncology Department of Surgery Department of Clinical Laboratory, People＇s Hospital of Gansu Province, Lanzhou 730000, China)
出处
《兰州大学学报（医学版）》
CAS
2017年第5期27-32,共6页
Journal of Lanzhou University（Medical Sciences）
基金
国家自然科学基金项目(81560498)
兰州市科技发展计划项目(2009-1-56)
