摘要
目的探讨Akt/mTOR信号通路在宫颈鳞癌组织中的表达及其临床意义。方法采用免疫组织化学SP法,检测20例正常宫颈组织、60例上皮内瘤变(CIN)组织和60例宫颈癌组织中蛋白激酶B(p-Akt)、哺乳动物雷帕霉素靶蛋白(p-mTOR)的表达,并分析其与临床病理特征之间的关系。结果正常宫颈组织、CIN和宫颈癌组织中p-Akt的表达阳性率分别为25.0%、46.67%、80.0%,宫颈癌组显著高于CIN组和正常组,差异有显著意义(χ~2=24.002,P<0.01)。p-mTOR在正常宫颈组织、CIN和宫颈癌组织中的表达阳性率分别为20.0%、55.0%、88.3%,各组比较差异有显著性(χ~2=36.859,P<0.05)。p-Akt、p-mTOR的表达与宫颈癌临床分期、淋巴结转移有关(χ~2=4.043~9.378,P<0.05),而与分化程度、术后复发无关(P>0.05)。宫颈鳞癌组织中p-Akt与p-mTOR蛋白的表达呈正相关(r=0.762,P<0.01)。结论 Akt/mTOR信号通路蛋白在宫颈癌组织中的阳性表达与肿瘤分期和淋巴结转移有关,有可能成为预测宫颈癌转移的新的分子标志物和治疗靶点。
Objective To investigate the expression of the Akt/mTOR（protein kinase B/mammalian target of rapamycin）signaling pathway in cervical squamous carcinoma tissues and its clinical significance. Methods The expression of p-Akt and p-mTOR in 20 cases of normal cervical epithelium tissues（normal group）,60 cases of cervical intraepithelial neoplasia（CIN）tissues（CIN group）,and 60 cases of cervical carcinoma tissues（cervical carcinoma group）were determined by immunohistochemical SP method,and then their relationships with clinicopathological factors were analyzed. Results The positive rates of p-Akt expression in the normal group,CIN group,and cervical carcinoma group were 25.0%,46.67%,and 80.0%,respectively,and the cervical carcinoma group had a significantly higher positive rate of p-Akt expression than the CIN group and the normal group（χ~2=24.002,P0.01）.There was a significant difference in the positive rate of p-mTOR expression between the normal group,the CIN group,and the cervical carcinoma group（20.0%,55.0%,and 88.3%;χ~2=36.859,P0.05）.The expression of p-Akt and p-mTOR was associated with the clinical stage of cervical carcinoma and lymph node metastasis（χ~2=4.043-9.378,P0.05）,but not with the degree of tumor differentiation and postoperative recurrence（P0.05）.There was a positive correlation between the expression of p-Akt and p-mTOR in cervical squamous cell carcinoma（r=0.762,P0.01）. Conclusion Positive expression of the Akt/mTOR signaling pathway in cervical squamous cell carcinoma tissues is associated with the tumor stage and lymph node metastasis,so it may serve as a new molecular marker for predicting the metastasis of cervical carcinoma and a therapeutic target.
出处
《齐鲁医学杂志》
2017年第4期388-390,共3页
Medical Journal of Qilu
基金
青岛市科技局基金资助项目(11-2-3-5-(1)-nsh)
