摘要
目的:探讨腺病毒介导CD269基因修饰树突状细胞(DC)的生物学特性及体外治疗多发性骨髓瘤(MM)的效果。方法:通过基因重组技术改建一种携B细胞成熟抗原(BCMA)CD269基因的无毒性腺相关病毒,感染DC后刺激产生杀伤MM细胞的特异性细胞毒性T淋巴细胞(CTLs)。采用流式细胞仪分析CTLs的γ干扰素(IFN-γ)表达情况,采用MTS实验检测CTLs对MM原代细胞的杀伤率及效靶比。结果:CTLs IFN-γ的表达率为29.07%,明显高于对照组的13.95%(P<0.05)。MTS实验表明,培养出来的CTLs对MM细胞株U266的杀伤率达到(32.53±2.92)%,对MM病人原代细胞的杀伤率为(28.23±3.11)%,而对正常病人原代细胞的杀伤率仅为(22.11±2.54)%。随着效靶比的升高,杀伤率增高。结论:实验所构建的CD269抗原病毒能刺激培养出针对MM的特异性杀伤细胞,为临床细胞治疗奠定了有效的实验基础。
Objective: To investigate the biological characteristics of adenovirus- mediated CD269 gene- modified dendritic cells and the effect of treatment of multiple myeloma (MM) in vitro. Methods: A non-toxic gonadal associated virus carrying B cell maturation antigen gene was constructed by gene recombination technique, and the infected dendritic cells were stimulated to produce specific cytotoxic T lymphocytes ( CTLs ) killing MM. The expression of IFN-'y in CTLs was analyzed by flow cytometry. The killing rate and effective target ratio of CTLs to primary myeloma cells were detected by MTS. Results : The expression rate of IFN-γ in CTLs was 29.07%, which was significantly higher than that in the control group ( 13.95%, P 〈 0.05 ). MTS experiments showed that the killing rate of the cultured CTLs for the multiple myeloma cell line U266 was (32.53± 2.92 ) %. And the killing rate of the primary cells of the patients with multiple myeloma was (28.23 ±3. 11 ) %. The killing rate of the cultured CTLs for normal primary cells was only (22. 11 ±2.54) %. With the increasing of the effect- to- target ratio, the killing rate increased. Conclusion: The CD269 antigen constructed by the experiment can stimulate the specific killing cells against the myeloma and provide an effective experimental basis for the clinical therapy.
出处
《现代医学》
2017年第10期1400-1404,共5页
Modern Medical Journal
基金
湖南省卫计委资助项目(C2015-75)