摘要
针对实时微型PCR荧光检测仪系统的核心-光学模块目前仍然存在系统灵敏度低、能量利用率低、聚焦光斑大等问题。文中采用在原共聚焦型光路系统基础上改进元器件的方法,利用全反射透镜收集准直光线,采用非球面透镜进行光线会聚,优化材料选择。搭建实验系统进行实验,实验结果表明,此系统解决了原系统存在的问题,检测灵敏度达到0.01 ng/m L(荧光素),比原系统提高了10倍,可在实际检测中使用。
There are still some problems in the core optical module of real time miniature PCR fluorescent detector system such as： low sensitivity of the system,low energy utilization rate,and big focal spot etc. Confocal optical system is still used in this paper and optimization designs of the original optical path system are adopted based on the fact of production; for instance,replacing the traditional lens with TIR Collimating Lens; focusing the optical path by using the aspheric lens,and improving the selection of lens materials. Finally,the test platform is built,experimental results verify that the sensitivity of the new system is increased by 10 times than before,it reaches 0.01 ng/ml（fluorescein） and the new system can be used in practical production.
出处
《电子科技》
2017年第11期34-37,共4页
Electronic Science and Technology
基金
国家重大科学仪器设备开发专项基金(2013YQ470765)
