摘要
目的探讨M2型巨噬细胞在食管癌细胞增殖、迁移、侵袭和上皮间质转化(epithelial-mesenchymal transition,EMT)中的作用。方法将人急性白血病单核细胞(THP-1)诱导分化为M2型巨噬细胞,然后将其与食管鳞癌Eca109和KYSE-150细胞系共培养,通过细胞增殖(MTT)、细胞划痕及细胞侵袭实验分别评价共培养细胞(共培养组)与非共培养细胞(非共培养组)增殖、迁移和侵袭能力的变化,并运用Western-blot检测EMT相关指标E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)和锌指转录因子(Slug)的表达变化。结果与非共培养组相比,共培养组食管鳞癌细胞迁移和侵袭能力明显增强(P<0.05),但其增殖能力差异无统计学意义(P>0.05)。Western-blot检测显示,上皮标志物E-cadherin表达减少,间质标志物Vimentin和Slug表达增加。结论 M2型巨噬细胞促进食管鳞癌细胞的迁移、浸润及上皮间质转化,但对其增殖无明显影响。
Objective To investigate the role of M2 macrophages in the proliferation,migration,invasion and epithelial-mesenchymal-transition(EMT)of ESCC cells.Methods Human acute monocytic leukemia cell line(THP-1)was artificially induced to differentiate into M2 macrophages,which co-cultured with ESCC Eca109 and KYSE-150 cell lines respectively.The proliferation,migration and invasion of ESCC cells were evaluated by MTT,wound-healing and Transwell invasive assay respectively.Epitehlial-mesenchymal transition(EMT)associated markers were measured by Western-blotting after co-culture with M2 macrophages.Results Compared with non-coculture group,M2 macrophages had significantly promotion of the migration and invasion abilities to ESCC cells(P<0.05)but no proliferation to ESCC cells(P>0.05).Western-blotting result reveals that the expression of E-cadherin,a typical epithelial marker,was down-regulated,while the expression of mesenchymal marker Vimentin and Slug were up-regulated.Conclusions M2 macrophages had significantly promoting effects on the migration and invasion to ESCC cells but no proliferation to the cell.In addition,M2 macrophages were also capable of inducing the EMT of ESCC cells.
出处
《新疆医科大学学报》
CAS
2017年第11期1393-1395,共3页
Journal of Xinjiang Medical University
基金
国家自然科学-新疆联合基金优秀青年项目(H1403522)
新疆维吾尔自治区自然科学基金面上项目(2015211C006)
