摘要
目的探讨电化学发光免疫法和酶联免疫法检测乙肝病毒标志物的检测效果。方法本研究选取了300例疑似乙型肝炎患者,按随机数字表法分为两组,对照组(150例)采用酶联免疫吸附法(ELISA)检测,观察组(150例)采用电化学发光免疫法检测。观察并记录两种方法对五种不同乙肝病毒标志物的检出率,对比不同浓度HBs Ag定值参比血清的最低检出浓度。结果观察组的HBs Ag和HBe Ab检出率分别为61.3%和24.0%,明显高于对照组的46.7%和13.3%(P<0.05),但两组HBs Ab,HBe Ag,HBc Ab的检出率比较,差异无统计学意义(P>0.05);取不同浓度HBs Ag定值做参比血清,用阴性血清进行倍比稀释,观察组的最低检出浓度均明显低于对照组(P<0.05)。结论电化学发光免疫法和酶联免疫吸附法和ELISA均能检测出乙肝病毒标志物,但电化学发光免疫法检测的灵敏度更高,能为临床提供更好的诊断依据。
Objective To study the effecl of eleclrochemiluminescence immunoassay and enzyme linked immunosorbenl assay （ ELISA） in lhe examining of HBV markers. Methods Three hundreds of suspected palienls with HBV were randomly divided into lwo groups. The control group （150 cases） was delected by ELISA, lhe observation group （150 cases） was measured by eleclrochemicalluminescence immunoassay. The detection rale of 5 different HBV markers and lhe minimum delectable concentration of reference serum for HBsAg al different concentrations were recorded and compared. Results The detection rale of HBsAg and HBeAb in lhe observation group were 61.3% and 24.0%, which were higher than 46.7% and 13.3% of lhe control group （ P 〈0.05）; bul there were no statistical significance on detection rale of HBsAb, HBeAg, HBcAb between lhe lwo groups （P 〉0.05）. Different concentrations of HBsAg constant reference serum were token and negative serum were used lo dilute them, lhe minimum detective concentration in lhe observation group was lower than lhal of lhe control （ P 〈0.05）. Conclusion Both lhe eleclrochemicalluminescence immunoassay and ELISA can detect lhe HBV makers, bul eleclrochemicalluminescence immunoassay is more sensitive than ELISA, which could provide belter information for clinical diagnosis.
作者
黄宁
张喆
马恺
HUANG Ning;ZHANG Zhe;MA Kai(Deparlmenl of Clinical Laboratory, Xixiang Chinese Medicine Hospital, Hanzhong 723500, Chin)
出处
《临床医学研究与实践》
2017年第5期132-133,共2页
Clinical Research and Practice
