摘要
在搅拌式生物反应器中 ,应用批培养、流加培养和灌流培养 3种方法对CHO -k1细胞进行了培养 .3种方法最大活细胞密度分别达到了 2 .0× 10 6,2 .7× 10 6和 9.6× 10 6mL-1.对培养过程中葡萄糖和氨基酸及代谢产物氨和乳酸的动态变化进行了检测和分析 .结果表明 ,CHO细胞对葡萄糖和谷氨酰胺的摄取以及氨和乳酸的产生与培养液中葡萄糖和谷氨酰胺的浓度正相关 ,培养过程中控制葡萄糖和谷氨酰胺的加入量能够明显降低氨和乳酸的产生 ,提高代谢效率 。
CHO-k1 cells were cultivated in stirred tank bioreactor.Batch culture,fed batch culture and perfusion culture were used,the maximuim viable cell density reached 2.0×10 6,2.7×10 6 and 9.6×10 6?mL -1 ,respectively.The concentration of nutrients (glucose and amino acid) and metabolic by products (ammonia and lactate) were analyzed during cultivation period.The relationship between cellular physiologic condition and nutrient metabolism is discussed in this paper.The energy metabolism in CHO-k1 cells is a typically substrate concentration-dependent overflow metabolism.The limitation of glucose and glutamine decreases by-product formation and increases metabolic efficiency.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
2002年第5期388-392,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省科技计划新药专项项目 (2 0 0 0XY0 5 )
