摘要
采用大孔吸附树脂对黑果枸杞中的原花青素粗提液进行纯化。以吸附能力和解吸附能力为指标,考察了AB-8,D130,D101,HPD100,D101-1和聚酰胺6种树脂对原花青素的纯化效果;以解吸能力为指标,考察洗脱剂体积分数、洗脱流速对洗脱效果的影响。结果表明,以D101树脂可用于黑果枸杞中原花青素的纯化,静态吸附以后,使用95%的乙醇,在2.5 BV/h的洗脱速度下,用4.0 BV进行洗脱,原花青素纯度由31.33%提高至68.03%;通过乙酸乙酯萃取可制得低聚原花青素样品,其平均聚合度由8.98降低至3.17,用HPLC方法可检测到低聚物中含有儿茶素、表儿茶素、原花青素B2等重要的原花青素单体和低聚物,根据峰面积计算三种物质的总含量达18.73%。
Macroporous resin were used to purify the crude proanthocynidins extract from Lycium ruthenicum Murr..Absorbation and desorption capacity were determined to study the purification effect on proanthpcyanidins of AB-8, D130, D101, HPD100, D101-1 and polyamide macroporous resin, then desorption capacity was used to evaluate the effect of eluant concentration and elution velocity for purifying.The results showed that D101 was better for purification among the supplied macroporous resins. After static absorbation, proanthocynidins was eluated by alcohol of 95% at the elution velocity of 2.5 BV/h and eluation volume of 4.0 BV, and the purity of proanthocynidins was enhanced from 31.33 % to 68.03 % by macroporous resin absorbation. After that,ethyl acetate was used to extract the oligomeric proanthocyanidins, and the DP was reduced from 8.98 to 3.17. Catechin,epicateehin and proanthocyanidin B2 were all detected in oligomeric proanthocyanidins by HPLC method, and their total content was 18.73% according to their peak area proportion.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第22期189-194,共6页
Science and Technology of Food Industry
基金
山东省优秀中青年科学家科研奖励基金(2014BSB01118)
关键词
黑果枸杞
原花青素
大孔树脂
纯化
Lycium ruthenicum Mutt.
proanthocyanidins
macroprous resin
purification
