摘要
通过溶剂热法制备出高磁性的聚合物微球MnFe_2O_4,经正硅酸乙酯(TEOS)和3-氨基丙基三乙氧基硅烷(APTS)对微球表面进行改性修饰,制备出新型复合材料MnFe_2O_4@SiO_2-NH_2,并将其用于固定青霉素G酰化酶。在Si/Fe比为7 mmol/g、n(TEOS)∶n(APTS)=1∶1时,固定化酶PGA/MnFe_2O_4@SiO_2-NH_2在37℃下水解青霉素G钾合成6-氨基青霉烷酸,表观酶活为1 660 IU/g、载酶量为107.1 mg/g、比酶活为15.5 IU/mg、活性回收率为46.9%。经过6次重复使用,保留初始酶活的81.3%,在使用中固定化酶在磁场的作用下能够快速沉降与产物分离。
High magnetic polymer microspheres MnFe2O4 were prepared by solvothermal method.The magnetic composite materials MnFe2O4@SiO2-NH2 were prepared by using tetraethoxysilane（ TEOS） and 3-aminopropyl triethoxysilane（ APTS） to decorating the surface of the microspheres MnFe2O4,and were used as the support for immobilization of penicillin G acylase（ PGA）.When the Si/Fe was 7 mmol/g and n（ TEOS） ∶ n（ APTS） = 1 ∶1,the apparent activity of PGA/MnFe2O4@ SiO2-NH2 for hydrolyzing penicillin G potassium into 6-aminopenicillanic acid at 37 ℃ was 1 660 IU/g,the enzyme load was about 107. 1 mg/g,the specific activity was 15. 5 IU/mg,and the activity recovery was about 46. 9% and retained the initial activity of 81. 3% after 6 recycles.The PGA/MnFe2O4@ Si O2-NH2 can settle quickly in the magnetic field and be separated easily from the product in applications.
出处
《化学试剂》
北大核心
2017年第11期1151-1154,共4页
Chemical Reagents
基金
国家自然科学基金资助项目(21663020)
关键词
磁性复合材料
固定化青霉素G酰化酶
催化活性
magnetic composite materials
immobilized penicillin G acylase
catalytic activity
